1. Cell Cycle/DNA Damage Epigenetics Autophagy
  2. Aurora Kinase Autophagy
  3. Reversine

Reversine  (Synonyms: 逆转素)

目录号: HY-14711 纯度: 99.22%
COA 产品使用指南

Reversine 是一种ATP-竞争性的 Aurora kinase 抑制剂,作用于 Aurora AAurora BAurora CIC50 分别为 400,500 和 400 nM。

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Reversine Chemical Structure

Reversine Chemical Structure

CAS No. : 656820-32-5

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥716
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1 mg ¥295
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5 mg ¥651
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10 mg ¥837
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50 mg ¥2325
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100 mg ¥4185
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500 mg   询价  

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Top Publications Citing Use of Products

    Reversine purchased from MCE. Usage Cited in: Onco Targets Ther. 2018 Feb 26;11:1025-1035.  [Abstract]

    Reversine inhibits the expression levels of Cyclin A2, Cyclin B1, and p-Rb, but promotes p21 expression.

    Reversine purchased from MCE. Usage Cited in: Onco Targets Ther. 2018 Feb 26;11:1025-1035.  [Abstract]

    Reversine inhibits the expression levels of Cyclin A2, Cyclin B1, and p-Rb, but promotes p21 expression.

    Reversine purchased from MCE. Usage Cited in: Chinese Pharmacological Bulletin, 2018, 34(12): 1740-1744.

    Western analysis of Cleaved-PARP, beta-tubulin, Bcl-2, Bcl-xL, Bax protein expression in the treatment of Reversine with different concentrations.

    查看 Aurora Kinase 亚型特异性产品:

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Reversine is a novel class of ATP-competitive Aurora kinase inhibitor with IC50s of 400, 500 and 400 nM for Aurora A, Aurora B and Aurora C, respectively.

    IC50 & Target[1]

    Aurora A

    400 nM (IC50)

    Aurora B

    500 nM (IC50)

    Aurora C

    400 nM (IC50)

    体外研究
    (In Vitro)

    Reversine 是一种新型极光激酶抑制剂,可抑制人急性髓性白血病细胞的集落形成。Reversine 是 Aurora A 和 B 的有效抑制剂,也是 Aurora C 激酶的抑制剂。浓度为 0.5 μM时,Aurora A 和 B 活性被抑制 80%,Aurora 激酶 C 被抑制 55%,而在其他测试的激酶上没有观察到抑制或仅观察到适度抑制。在第二轮实验中,Reversine 对极光激酶 A 的 IC50 测定为 400 nM,而极光激酶 B 和 C IC50 分别为 500 和 400 nM。MEK1 的 IC50 也测定为 >1.5 μM,肌肉肌球蛋白 (非肌肉肌球蛋白 II 的类似物) 的 IC50 为 350 nM[1]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    体内研究
    (In Vivo)

    Reversine 和 Aspirin 的组合可以更有效地诱导细胞周期停滞和细胞凋亡。为了评估这种组合的抗肿瘤作用,通过皮下注射建立了异种移植裸鼠模型。接种宫颈癌细胞的小鼠在肿瘤接种后约 16 天时体重减轻了约 10%。然而,联合处理组的肿瘤生长 (肿瘤重量) 减少,小鼠存活时间更长[2]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    分子量

    393.49

    Formula

    C21H27N7O

    CAS 号
    性状

    固体

    颜色

    Light green to green

    中文名称

    逆转素

    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    溶解性数据
    In Vitro: 

    DMSO 中的溶解度 : 20 mg/mL (50.83 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 2.5414 mL 12.7068 mL 25.4136 mL
    5 mM 0.5083 mL 2.5414 mL 5.0827 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 1 years; -20°C, 6 months。-80°C储存时,请在1年内使用, -20°C储存时,请在6个月内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    纯度 & 产品资料

    纯度: 99.22%

    参考文献
    Cell Assay
    [1]

    HCT116 and HL60 cells are incubated with either 5 μmol/L Reversine or DMSO 0.01%. Cells are harvested and fixed in 70% ethanol overnight. After double washing with PBS, cells are labeled with cell cycle staining reagent PBS, 0.1% Triton X-100, 200 μg/mL DNase-free RNase, and 25 μg/mL propidium iodide and incubated at room temperature in the dark for 30 min. DNA content is analyzed using FACSCalibur. Cell viability of different tumor cell lines is assessed using ATPlite 1step. Briefly, 2×104 cells for each well are plated in a 96-well plate in presence of crescent quantity of Reversine. After 72 h, the plates are recovered and 100 μL ATPlite solution is added to each well. The plates are shaken for 2 min at 700 rpm and luminescence is measured using EnVision Multilabel plate reader. Each sample is analyzed in triplicate[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [2]

    Mice[2]
    Female athymic 6-8 weeks old BALB/c nude mice are used. U14 cell suspension (5×106 cells in 100 μL of RPMI-1640 medium) is injected subcutaneously. The purpose of developing cervical tumors is to generate histological intact tumors for drug therapy. When the diameter of tumors reached up to about 1 cm, Reversine, aspirin or their combinations are administrated by intraperitoneal injection per 3 days, twenty-five of these mice are randomly assigned into one of the following five groups: (a) mice treated with RPMI-1640 medium, (b) mice treated with DMSO, (c) mice treated with Reversine (10 mg/kg), (d) mice treated with aspirin (1 μg/kg) and (e) mice treated with a Reversine and aspirin combination. Body weight and tumor size at the site of inoculation are measured three times a week. Tumor size is measured every 3 days from two diameters, tumor volume is estimated using the formula L×S2/2(L as the longest diameter, S as the shortest diameter).

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    参考文献

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 1 years; -20°C, 6 months。-80°C储存时,请在1年内使用, -20°C储存时,请在6个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.5414 mL 12.7068 mL 25.4136 mL 63.5340 mL
    5 mM 0.5083 mL 2.5414 mL 5.0827 mL 12.7068 mL
    10 mM 0.2541 mL 1.2707 mL 2.5414 mL 6.3534 mL
    15 mM 0.1694 mL 0.8471 mL 1.6942 mL 4.2356 mL
    20 mM 0.1271 mL 0.6353 mL 1.2707 mL 3.1767 mL
    25 mM 0.1017 mL 0.5083 mL 1.0165 mL 2.5414 mL
    30 mM 0.0847 mL 0.4236 mL 0.8471 mL 2.1178 mL
    40 mM 0.0635 mL 0.3177 mL 0.6353 mL 1.5884 mL
    50 mM 0.0508 mL 0.2541 mL 0.5083 mL 1.2707 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    产品名称:
    Reversine
    目录号:
    HY-14711
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