2. PI3K/Akt/mTOR
  3. Akt
  4. Crosstide

Crosstide 是一种糖原合成酶激酶α/β融合蛋白序列的模拟肽，是 Akt 的底物。

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Custom Peptide Synthesis

Crosstide Chemical Structure

Crosstide Chemical Structure

CAS No. : 171783-05-4

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规格 价格 是否有货 数量
1 mg ￥1100
In-stock
5 mg ￥3300
In-stock
10 mg ￥5115
In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Customer Review

Other Forms of Crosstide:

Crosstide 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 1 篇科研文献

查看 Akt 亚型特异性产品:

查看所有亚型
Akt Akt1 Akt2 Akt3

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Crosstide is a peptide analog of glycogen synthase kinase α/β fusion protein sequence which is a substrate for Akt.

体外研究
(In Vitro)

16HBE14o− cells exposed to RV39 demonstrate a crosstide kinase activity in vitro. Serine phosphorylation of crosstide is confirmed by immunoblotting, and phosphorylation is blocked by PP2 but not by PP3[1]. The wildtype GST-AKT2 shows significant phosphoryl transferase activity towards crosstide, reaching an initial velocity of 16 pmol phosphate/min/μg enzyme. The mutant GST-AKT2T/E,S/D displayed an initial velocity of 85 pmol phosphate/min/μg kinase for phosphorylation of Crosstide, corresponding to a 5-fold increase compared to the wildtype enzyme[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Crosstide 相关抗体:
分子量

1164.23

Formula

C48H77N17O17
CAS 号
性状

固体

颜色

White to off-white

Sequence

Gly-Arg-Pro-Arg-Thr-Ser-Ser-Phe-Ala-Glu-Gly
Sequence Shortening

GRPRTSSFAEG
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O 中的溶解度 : ≥ 50 mg/mL (42.95 mM)

* "≥" means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.8589 mL 4.2947 mL 8.5894 mL
5 mM 0.1718 mL 0.8589 mL 1.7179 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
纯度 & 产品资料
参考文献
Kinase Assay
[1]

After serum deprivation for 24 h, cells are incubated with digoxigenin-labeled sham protein or digoxigenin-labeled RV39 at an MOI of 1.0 for 10 min. Cell homogenates are immunoprecipitated with mouse anti-digoxigenin antibody and precipitates incubated with Crosstide and [γ-32P]ATP. Crosstide is a glycogen synthase kinase α/β fusion protein sequence (GRPRTSSFAEG) which is a substrate for Akt. Samples are processed for autoradiography and immunoblotting using rabbit anti-phospho-Tyr416 Src, mouse anti-Src (clone GD11), rabbit anti-phospho-Ser473, or rabbit anti-Akt.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

Crosstide 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.8589 mL 4.2947 mL 8.5894 mL 21.4734 mL
5 mM 0.1718 mL 0.8589 mL 1.7179 mL 4.2947 mL
10 mM 0.0859 mL 0.4295 mL 0.8589 mL 2.1473 mL
15 mM 0.0573 mL 0.2863 mL 0.5726 mL 1.4316 mL
20 mM 0.0429 mL 0.2147 mL 0.4295 mL 1.0737 mL
25 mM 0.0344 mL 0.1718 mL 0.3436 mL 0.8589 mL
30 mM 0.0286 mL 0.1432 mL 0.2863 mL 0.7158 mL
40 mM 0.0215 mL 0.1074 mL 0.2147 mL 0.5368 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Crosstide171783-05-4AktPKBProtein kinase BInhibitorinhibitorinhibit

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产品名称：
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目录号：
HY-P0315
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