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  3. SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy

SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy

  • Nat Commun. 2015 Oct 1:6:8497. doi: 10.1038/ncomms9497.
Gražvydas Lukinavičius 1,  Claudia Blaukopf 2,  Elias Pershagen 3,  Alberto Schena 1,  Luc Reymond 1,  Emmanuel Derivery 4,  Marcos Gonzalez-Gaitan 4,  Elisa D'Este 5,  Stefan W Hell 5,  Daniel Wolfram Gerlich 2,  Kai Johnsson 1
Affiliations

Affiliations

  • 1 Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, NCCR in Chemical Biology, 1015 Lausanne, Switzerland.
  • 2 Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter Campus (VBC), Dr Bohr Gasse 3, 1030 Vienna, Austria.
  • 3 Department of Chemistry-BMC, Uppsala University, 75123 Uppsala, Sweden.
  • 4 Department of Biochemistry, NCCR in Chemical Biology, University of Geneva, 1211 Geneva, Switzerland.
  • 5 Department NanoBiophotonics, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.
PMID: 26423723 DOI: 10.1038/ncomms9497
Abstract

Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue LIGHT or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA Stain, SiR-Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy. The combination of these properties makes this probe a powerful tool for live-cell imaging.

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