1. Academic Validation
  2. Targeting WEE1 to enhance conventional therapies for acute lymphoblastic leukemia

Targeting WEE1 to enhance conventional therapies for acute lymphoblastic leukemia

  • J Hematol Oncol. 2018 Aug 1;11(1):99. doi: 10.1186/s13045-018-0641-1.
Andrea Ghelli Luserna Di Rorà 1 Neil Beeharry 2 3 Enrica Imbrogno 4 Anna Ferrari 4 Valentina Robustelli 4 Simona Righi 4 Elena Sabattini 4 Maria Vittoria Verga Falzacappa 5 Chiara Ronchini 5 Nicoletta Testoni 4 Carmen Baldazzi 4 Cristina Papayannidis 4 Maria Chiara Abbenante 4 Giovanni Marconi 4 Stefania Paolini 4 Sarah Parisi 4 Chiara Sartor 4 Maria Chiara Fontana 4 Serena De Matteis 6 Ilaria Iacobucci 4 7 Pier Giuseppe Pelicci 5 Michele Cavo 4 Timothy J Yen 2 Giovanni Martinelli 8
Affiliations

Affiliations

  • 1 Department of Experimental, Diagnostic and Specialty Medicine, Institute of Hematology "L. e A. Seràgnoli", University of Bologna, Via Massarenti 9, 40138, Bologna, Italy. andrea.ghelliluserna@studio.unibo.it.
  • 2 Cancer Biology Program, Fox Chase Cancer Center, Philadelphia, PA, USA.
  • 3 LAM Therapeutics, Guilford, CT, USA.
  • 4 Department of Experimental, Diagnostic and Specialty Medicine, Institute of Hematology "L. e A. Seràgnoli", University of Bologna, Via Massarenti 9, 40138, Bologna, Italy.
  • 5 Laboratory of Clinical Genomics, European Institute of Oncology, Milan, Italy.
  • 6 Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy.
  • 7 Department of Pathology, St. Jude Children's Research Hospital, Memphis, TN, USA.
  • 8 Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy.
Abstract

Background: Despite the recent progress that has been made in the understanding and treatment of acute lymphoblastic leukemia (ALL), the outcome is still dismal in adult ALL cases. Several studies in solid tumors identified high expression of Wee1 kinase as a poor prognostic factor and reported its role as a cancer-conserving oncogene that protects Cancer cells from DNA damage. Therefore, the targeted inhibition of Wee1 kinase has emerged as a rational strategy to sensitize Cancer cells to antineoplastic compounds, which we evaluate in this study.

Methods: The effectiveness of the selective Wee1 Inhibitor AZD-1775 as a single agent and in combination with different antineoplastic agents in B and T cell precursor ALL (B/T-ALL) was evaluated in vitro and ex vivo studies. The efficacy of the compound in terms of cytotoxicity, induction of Apoptosis, and changes in gene and protein expression was assessed using different B/T-ALL cell lines and confirmed in primary ALL blasts.

Results: We showed that Wee1 was highly expressed in adult primary ALL bone marrow and peripheral blood blasts (n = 58) compared to normal mononuclear cells isolated from the peripheral blood of healthy donors (p = 0.004). Thus, we hypothesized that Wee1 could be a rational target in ALL, and its inhibition could enhance the cytotoxicity of conventional therapies used for ALL. We evaluated the efficacy of AZD-1775 as a single agent and in combination with several antineoplastic agents, and we elucidated its mechanisms of action. AZD-1775 reduced cell viability in B/T-ALL cell lines by disrupting the G2/M checkpoint and inducing Apoptosis. These findings were confirmed in human primary ALL bone marrow and peripheral blood blasts (n = 15). In both cell lines and primary leukemic cells, AZD-1775 significantly enhanced the efficacy of several tyrosine kinase inhibitors (TKIs) such as bosutinib, imatinib, and ponatinib, and of chemotherapeutic agents (clofarabine and doxorubicin) in terms of the reduction of cell viability, Apoptosis induction, and inhibition of proliferation.

Conclusions: Our data suggest that Wee1 plays a role in ALL blast's survival and is a bona fide target for therapeutic intervention. These data support the evaluation of the therapeutic potential of AZD-1775 as chemo-sensitizer agent for the treatment of B/T-ALL.

Keywords

Acute lymphoblastic leukemia; Chemo-sensitizer agent; G2/M checkpoint; WEE1 inhibitor.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-10993
    99.97%, Wee1抑制剂