1. Academic Validation
  2. Autologous decellularized extracellular matrix protects against H2O2-induced senescence and aging in adipose-derived stem cells and stimulates proliferation in vitro

Autologous decellularized extracellular matrix protects against H2O2-induced senescence and aging in adipose-derived stem cells and stimulates proliferation in vitro

  • Biosci Rep. 2019 May 21;39(5):BSR20182137. doi: 10.1042/BSR20182137.
Xiaofang Yu 1 Yucang He 1 Zhuojie Chen 1 Yao Qian 1 Jingping Wang 1 Ziwan Ji 1 Xiaoyan Tan 2 Liqun Li 3 Ming Lin 4
Affiliations

Affiliations

  • 1 First Affiliated Hospital of Wenzhou Medical University, Nanbaixiang, Ouhai Direct, Wenzhou City, Zhejiang Province, People's Republic of China.
  • 2 Hangzhou plastic surgery hospital, Xiacheng Direct, Hangzhou City, Zhejiang Province, People's Republic of China.
  • 3 First Affiliated Hospital of Wenzhou Medical University, Nanbaixiang, Ouhai Direct, Wenzhou City, Zhejiang Province, People's Republic of China wz.llq@163.com dr.lm@163.com.
  • 4 Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, West Xueyuan Road, Lucheng Direct, Wenzhou City, Zhejiang Province, People's Republic of China wz.llq@163.com dr.lm@163.com.
Abstract

Background: Adipose-derived stem cells have attracted significant interest, especially in stem cell therapy and regenerative medicine. However, these cells undergo gradual premature senescence in long-term cultures, which are essential for clinical applications that require cell-assisted lipotransfer or tissue repair. Methods: Since the extracellular matrix forms the microenvironment around stem cells in vitro and regulates self-renewal and multipotency in part by slowing down stem cell aging, we evaluated its potential to protect against senescence, using H2O2-induced adipose-derived stem cells as a model. Results: We found that supplementing cultures with decellularized extracellular matrix harvested from the same cells significantly promotes proliferation and reverses signs of senescence, including decreased multipotency, increased expression of senescence-associated β-galactosidase, and accumulation of Reactive Oxygen Species. Conclusion: These findings suggest a novel approach in which an autologous decellularized extracellular matrix is used to prevent cellular senescence to enable the use of adipose-derived stem cells in regenerative medicine.

Keywords

Adipose-derived stem cells; extracellular matrix; hydrogen peroxide; lipotransfer; multipotency; senescence.

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