1. Academic Validation
  2. Crinamine Induces Apoptosis and Inhibits Proliferation, Migration, and Angiogenesis in Cervical Cancer SiHa Cells

Crinamine Induces Apoptosis and Inhibits Proliferation, Migration, and Angiogenesis in Cervical Cancer SiHa Cells

  • Biomolecules. 2019 Sep 16;9(9):494. doi: 10.3390/biom9090494.
Phattharachanok Khumkhrong 1 Kitiya Piboonprai 1 Waraluck Chaichompoo 2 Wittaya Pimtong 1 Mattaka Khongkow 1 Katawut Namdee 1 Angkana Jantimaporn 1 Deanpen Japrung 1 Udom Asawapirom 1 Apichart Suksamrarn 2 Tawin Iempridee 3
Affiliations

Affiliations

  • 1 National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency, Pathum Thani 12120, Thailand.
  • 2 Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Ramkhamhaeng University, Bangkok 10240, Thailand.
  • 3 National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency, Pathum Thani 12120, Thailand. tawin@nanotec.or.th.
Abstract

Crinumasiaticum is a perennial herb widely distributed in many warmer regions, including Thailand, and is well-known for its medicinal and ornamental values. Crinum Alkaloids contain numerous compounds, such as crinamine. Even though its mechanism of action is still unknown, crinamine was previously shown to possess Anticancer activity. In this study, we demonstrate that crinamine was more cytotoxic to cervical Cancer cells than normal cells. It also inhibited anchorage-independent tumor spheroid growth more effectively than existing chemotherapeutic drugs carboplatin and 5-fluorouracil or the CDK9 Inhibitor FIT-039. Additionally, unlike cisplatin, crinamine induced Apoptosis without promoting DNA double-strand breaks. It suppressed cervical Cancer cell migration by inhibiting the expression of positive regulators of epithelial-mesenchymal transition SNAI1 and VIM. Importantly, crinamine also exerted anti-angiogenic activities by inhibiting secretion of VEGF-A protein in cervical Cancer cells and blood vessel development in zebrafish embryos. Gene expression analysis revealed that its mechanism of action might be attributed, in part, to downregulation of cancer-related genes, such as Akt1, BCL2L1, CCND1, CDK4, PLK1, and RHOA. Our findings provide a first insight into crinamine's Anticancer activity, highlighting its potential use as an alternative bioactive compound for cervical Cancer chemoprevention and therapy.

Keywords

Crinum asiaticum; cervical cancer; crinamine; tumor spheroid; zebrafish.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-12871B
    99.80%, PTEFb/CDK9 抑制剂
    CDK