1. Academic Validation
  2. Melatonin attenuates manganese-induced mitochondrial fragmentation by suppressing the Mst1/JNK signaling pathway in primary mouse neurons

Melatonin attenuates manganese-induced mitochondrial fragmentation by suppressing the Mst1/JNK signaling pathway in primary mouse neurons

  • Sci Total Environ. 2022 Oct 20;844:157134. doi: 10.1016/j.scitotenv.2022.157134.
Zhuo-Fan Liu 1 Kuan Liu 1 Zhi-Qi Liu 1 Lin Cong 1 Meng-Yu Lei 1 Jing Li 1 Zhuo Ma 1 Yu Deng 1 Wei Liu 1 Bin Xu 2
Affiliations

Affiliations

  • 1 Department of Environmental Health, School of Public Health, China Medical University, China.
  • 2 Department of Environmental Health, School of Public Health, China Medical University, China. Electronic address: bxu10@cmu.edu.cn.
Abstract

Manganese (Mn) toxicity is mainly caused by excessive Mn content in drinking water and occupational exposure. Moreover, overexposure to Mn can impair mental, cognitive, memory, and motor capacities. Although melatonin (Mel) can protect against Mn-induced neuronal damage and mitochondrial fragmentation, the underlying mechanism remains elusive. Here, we examined the related molecular mechanisms underlying Mel attenuating Mn-induced mitochondrial fragmentation through the mammalian sterile 20-like kinase-1 (Mst1)/JNK signaling path. To test the role of Mst1 in mitochondrial fragmentation, we treated mouse primary neurons overexpressing Mst1 with Mel and Mn stimulation. In normal neurons, 10 μM Mel reduced the effects of Mn (200 μM) on Mst1 expression at the mRNA and protein levels and on phosphorylation of JNK and Drp1, Drp1 mitochondrial translocation, and mitochondrial fragmentation. Conversely, overexpression of Mst1 hindered the protective effect of Mel (10 μM) against Mn-induced mitochondrial fragmentation. Anisomycin (ANI), an activator of JNK signaling, was similarly found to inhibit the protective effect of Mel on mitochondria, while Mst1 levels were not significantly changed. Thus, our results demonstrated that 10 μM Mel negatively regulated the Mst1-JNK pathway, thereby reducing excessive mitochondrial fission, maintaining the mitochondrial network, and alleviating Mn-induced mitochondrial dysfunction.

Keywords

C-Jun NH2 terminal kinase; Dynamin-related protein 1; Mammalian sterile 20-like kinase-1; Mel; Mitochondrial fission; Mn.

Figures
Products