2. Academic Validation
  3. Cell-type-specific PtrWOX4a and PtrVCS2 form a regulatory nexus with a histone modification system for stem cambium development in Populus trichocarpa

Cell-type-specific PtrWOX4a and PtrVCS2 form a regulatory nexus with a histone modification system for stem cambium development in Populus trichocarpa

  • Nat Plants. 2023 Jan 9. doi: 10.1038/s41477-022-01315-7.
Xiufang Dai 1,  Rui Zhai 1,  Jiaojiao Lin 1,  Zhifeng Wang 1 2,  Dekai Meng 1,  Meng Li 1,  Yuli Mao 1,  Boyuan Gao 1,  Hongyan Ma 1,  Baofeng Zhang 1,  Yi Sun 1,  Shuang Li 1,  Chenguang Zhou 1,  Ying-Chung Jimmy Lin 1 3,  Jack P Wang 1 4,  Vincent L Chiang 1 4,  Wei Li 5
Affiliations

Affiliations

  • 1 State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.
  • 2 National Key Laboratory of Plant Molecular Genetics and CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.
  • 3 Department of Life Sciences and Institute of Plant Biology, College of Life Science, National Taiwan University, Taipei, Taiwan, China.
  • 4 Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC, USA.
  • 5 State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. weili2015@nefu.edu.cn.
PMID: 36624255 DOI: 10.1038/s41477-022-01315-7
Abstract

Stem vascular cambium cells in forest trees produce wood for Materials and energy. WOX4 affects the proliferation of such cells in Populus. Here we show that PtrWOX4a is the most highly expressed stem vascular-cambium-specific (VCS) gene in P. trichocarpa, and its expression is controlled by the product of the second most highly expressed VCS gene, PtrVCS2, encoding a zinc finger protein. PtrVCS2 binds to the PtrWOX4a promoter as part of a PtrWOX13a-PtrVCS2-PtrGCN5-1-PtrADA2b-3 protein tetramer. PtrVCS2 prevented the interaction between PtrGCN5-1 and PtrADA2b-3, resulting in H3K9, H3K14 and H3K27 hypoacetylation at the PtrWOX4a promoter, which led to fewer cambium cell layers. These effects on cambium cell proliferation were consistent across more than 20 sets of transgenic lines overexpressing individual genes, gene-edited mutants and RNA interference lines in P. trichocarpa. We propose that the tetramer-PtrWOX4a system may coordinate genetic and epigenetic regulation to maintain normal vascular cambium development for wood formation.

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