2. Academic Validation
  3. Generation of vascularized human cardiac organoids for 3D in vitro modeling

Generation of vascularized human cardiac organoids for 3D in vitro modeling

  • STAR Protoc. 2023 Jun 28;4(3):102371. doi: 10.1016/j.xpro.2023.102371.
Holly K Voges 1,  Richard J Mills 2,  Enzo R Porrello 3,  James E Hudson 4
Affiliations

Affiliations

  • 1 QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006 Australia; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, VIC 3052 Australia; Department of Paediatrics, School of Medicine, Dentistry and Health Sciences, The University of Melbourne, Melbourne, VIC 3052, Australia; School of Biomedical Sciences, The University of Queensland, Brisbane, QLD 4072, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine, Murdoch Children's Research Institute, Melbourne, VIC 3052, Australia. Electronic address: holly.voges@mcri.edu.au.
  • 2 QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006 Australia; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, VIC 3052 Australia; Department of Paediatrics, School of Medicine, Dentistry and Health Sciences, The University of Melbourne, Melbourne, VIC 3052, Australia; School of Biomedical Sciences, The University of Queensland, Brisbane, QLD 4072, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine, Murdoch Children's Research Institute, Melbourne, VIC 3052, Australia; School of Biomedical Sciences, Queensland University of Technology, Kelvin Grove, QLD 4000, Australia.
  • 3 Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, VIC 3052 Australia; Novo Nordisk Foundation Center for Stem Cell Medicine, Murdoch Children's Research Institute, Melbourne, VIC 3052, Australia; Department of Anatomy and Physiology, School of Biomedical Sciences, The University of Melbourne, Melbourne, VIC 3052, Australia; Melbourne Centre for Cardiovascular Genomics and Regenerative Medicine, The Royal Children's Hospital, Melbourne, VIC 3052, Australia.
  • 4 QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006 Australia; School of Biomedical Sciences, The University of Queensland, Brisbane, QLD 4072, Australia. Electronic address: james.hudson@qimrberghofer.edu.au.
PMID: 37384522 DOI: 10.1016/j.xpro.2023.102371
Abstract

Here, we provide a protocol for next-generation human cardiac organoid modeling containing markers of vascularized tissues. We describe steps for cardiac differentiation, harvesting cardiac cells, and generating vascularized human cardiac organoids. We then detail downstream analysis of functional parameters and fluorescence labeling of human cardiac organoids. This protocol is useful for high throughput disease modeling, drug discovery, and providing mechanistic insight into cell-cell and cell-matrix interactions. For complete details on the use and execution of this protocol, please refer to Voges et al.1 and Mills et al.2.

Keywords

Cell Differentiation; Cell culture; Tissue Engineering.

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