IL-36 gamma/IL-1F9 蛋白, Human (152a.a)

IL-36 gamma (IL-1F9), a subform of IL-36 family, belongs to IL-1 superfamily. IL-36 gamma mediates inflammatory response. L-36 beta binds to IL-36R and recruits the co-receptor IL-1RacP, and thereby activating NF-κB and MAPK signaling pathways, but the activation requires N-terminal cleavage by neutrophil granule-derived proteases^[1][2]. IL-36 gamma/IL-1F9 Protein, Human (152a.a) is a recombinant human IL-36 gamma (S18-D169) without any tag, which is produced in E. coli.

IL-36γ（IL-1F9）是IL-36家族的一个亚型，属于IL-1超家族。IL-36 γ 表达于外周血淋巴细胞、角质形成细胞、支气管上皮细胞和 THP-1 细胞^[3]。
不同物种的 IL-36 γ 氨基酸序列不同。人 IL-36 gamma 与小鼠的氨基酸序列同一性<55%。
IL-36 γ具有β-三叶结构。L-36 γ 与 IL-36R 结合并招募共受体 IL-1RAcP。因此，异二聚体信号复合物使 2 条受体链的 Toll/IL-1R (TIR) 结构域非常接近，从而激活 NF-κB 和 MAPK 信号通路^[1]。但激活需要中性粒细胞颗粒衍生的蛋白酶（例如组织蛋白酶 G、弹性蛋白酶和蛋白酶-3^{[1][2）在 Val1518 的 N 端裂解]}。IL-36 γ 与慢性炎症和癌症有关。IL-36 γ 在皮肤银屑病病变、先天性巨结肠患者的结肠无神经节和神经节区域以及炎症性肠病 (IBD) 患者的发炎粘膜中表达上调^[1][4]。
IL-36 γ 是一种促炎因子。IL-36 gamma 通过激活 NF-κB 和 MAPK 信号通路介导炎症反应^[2]。

IL-36 gamma (human, 50 ng/mL for 4 h or 100 ng/mL for 24 h) promotes the expression of pro-inflammatory cytokines and antibacterial peptides in NHOKs (oral keratinocytes)^[5].
IL-36 gamma (human, 0.1 ng/mL, 3 days) promotes colony formation, migration and invasion of AGS cells^[6].

The ability to induce IL-8 secretion in A431 human epithelial carcinoma cells has an ED₅₀ value of 5-20 ng/mL.

Human

E. coli

Tag Free

Q9NZH8 (S18-D169)

56300  [NCBI]

SMCKPITGTINDLNQQVWTLQGQNLVAVPRSDSVTPVTVAVITCKYPEALEQGRGDPIYLGIQNPEMCLYCEKVGEQPTLQLKEQKIMDLYGQPEPVKPFLFYRAKTGRTSTLESVAFPDWFIASSKRDQPIILTSELGKSYNTAFELNIND

14-16 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 0.1 mM EDTA, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Bassoy EY, et al. Regulation and function of interleukin-36 cytokines. Immunol Rev. 2018 Jan;281(1):169-178.  [Content Brief]

  [2]. Zhou L,et al. Interleukin-36: Structure, Signaling and Function. Adv Exp Med Biol. 2021;21:191-210.  [Content Brief]

  [3]. Gresnigt MS, et al. Biology of IL-36 cytokines and their role in disease. Semin Immunol. 2013 Dec 15;25(6):458-65.  [Content Brief]

  [4]. Neurath MF. IL-36 in chronic inflammation and cancer. Cytokine Growth Factor Rev. 2020 Oct;55:70-79.  [Content Brief]

  [5]. Xu P, et al. The mechanism on Prevotella melaninogenica promoting the inflammatory progression of oral lichen planus. Clin Exp Immunol. 2022 Aug 19;209(2):215-224.  [Content Brief]

  [6]. Le N, Luk I, et al. IL-36G promotes cancer-cell intrinsic hallmarks in human gastric cancer cells. Cytokine. 2022 Jul;155:155887.  [Content Brief]