1. 重组蛋白
  2. Receptor Proteins
  3. Pattern Recognition Receptors
  4. C-type Lectin Receptors
  5. LOX-1
  6. LOX-1/OLR1 蛋白, Rat (HEK293, Fc)

LOX-1/OLR1 蛋白, Rat (HEK293, Fc)

目录号: HY-P73836
产品使用指南

LOX-1/OLR1 蛋白作为血管内皮细胞中识别、内化和降解氧化修饰低密度脂蛋白 (oxLDL)(动脉粥样硬化标志物)的受体发挥着重要作用。OxLDL 会诱导内皮功能障碍,引发促炎症反应、氧化条件和细胞凋亡。LOX-1/OLR1 蛋白, Rat (HEK293, Fc) 是重组的 LOX-1/OLR1 蛋白,由 HEK293 表达,带有 N-hFc 标签。LOX-1/OLR1 蛋白, Rat (HEK293, Fc) 全长 305 个氨基酸,分子量分别约为 ~66 & 35 kDa。

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描述

The LOX-1/OLR1 protein plays an important role as a receptor that recognizes, internalizes, and degrades oxidatively modified low-density lipoprotein (oxLDL), a marker of atherosclerosis, in vascular endothelial cells. OxLDL induces endothelial dysfunction, triggering pro-inflammatory responses, oxidative conditions, and apoptosis. LOX-1/OLR1 Protein, Rat (HEK293, Fc) is the recombinant rat-derived LOX-1/OLR1 protein, expressed by HEK293 , with N-hFc labeled tag. The total length of LOX-1/OLR1 Protein, Rat (HEK293, Fc) is 305 a.a., with molecular weight of ~66 & 35 kDa, respectively.

研究背景

LOX-1/OLR1 蛋白作为受体,在血管内皮细胞对氧化修饰低密度脂蛋白 (oxLDL) 的识别、内化和降解中发挥着至关重要的作用。OxLDL 是动脉粥样硬化的标志物,可诱导内皮细胞活化和功能障碍,导致促炎症反应、促氧化条件和细胞凋亡。与 oxLDL 的结合会触发 NF-kappa-B 的激活,导致细胞内活性氧产生增加和各种促动脉粥样硬化细胞反应,包括减少一氧化氮释放、单核细胞粘附和细胞凋亡。除了在脂质代谢中的作用外,LOX-1/OLR1 还充当 HSP70 的受体,促进抗原交叉呈递给树突状细胞中的幼稚 T 细胞,并参与细胞介导的抗原交叉呈递。此外,它还参与炎症过程,在内毒素诱导的炎症过程中充当血管界面上的白细胞粘附分子。此外,LOX-1/OLR1 还可作为晚期糖基化终末产物、活化血小板、单核细胞、凋亡细胞以及革兰氏阴性和革兰氏阳性细菌的受体。它形成同型二聚体,可能组织成由三个同型二聚体组成的六聚体,并与 HSP70 相互作用。

种属

Rat

表达系统

HEK293

标签

N-hFc

蛋白编号

O70156 (L60-Q364)

基因 ID

140914  [NCBI]

蛋白结构
N-term
hFc
LOX-1 (L60-Q364)
Accession # O70156
C-term
同用名
Oxidized low-density lipoprotein receptor 1; LOX-1; OLR1; CLEC8A
分子量

Approximately 66&35 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

LOX-1/OLR1 Protein, Rat (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
LOX-1/OLR1 Protein, Rat (HEK293, Fc)
目录号:
HY-P73836
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