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  4. SMPD1 蛋白, Mouse (sf9, His)

SMPD1 蛋白, Mouse (sf9, His)

目录号: HY-P76134
产品使用指南

SMPD1 蛋白通过两种酶促形式将鞘磷脂转化为神经酰胺，一种靶向内溶酶体，另一种则释放到细胞外。溶酶体胞吐作用成为应激反应期间分泌形式的重要来源。SMPD1 还从内溶酶体内膜输出胆固醇并表现出磷脂酶 C 活性。它在应激诱导的细胞凋亡调节中的作用是通过神经酰胺的产生介导的。SMPD1 蛋白, Mouse (sf9, His) 是重组的 SMPD1 蛋白，由 Sf9 insect cells 表达，带有 C-His 标签。SMPD1 蛋白, Mouse (sf9, His) 全长 626 个氨基酸，分子量约为 ~63 KDa。

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描述

The SMPD1 protein converts sphingomyelin to ceramide through two enzymatic forms, one targeted to endolysosomes and the other released extracellularly. Lysosomal exocytosis becomes a significant source of the secretory form during stress responses. SMPD1 also exports cholesterol from intraendolysosomal membranes and exhibits phospholipase C activities. Its role in stress-induced apoptosis modulation is mediated through ceramide production. SMPD1 Protein, Mouse (sf9, His) is the recombinant mouse-derived SMPD1 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of SMPD1 Protein, Mouse (sf9, His) is 626 a.a., with molecular weight of ~63 KDa.
研究背景

SMPD1 蛋白的功能是通过两种酶促形式将鞘磷脂转化为神经酰胺，这两种酶促形式源自单一前体蛋白的替代运输。一种形式靶向内溶酶体区室，而另一种形式则释放到细胞外。然而，在应激反应期间，溶酶体胞吐作用可能作为分泌形式的重要来源。在溶酶体中，它执行鞘磷脂向神经酰胺的转化，并在内溶酶体内膜输出胆固醇方面发挥着至关重要的作用。此外，SMPD1 对 1,2-二酰基甘油磷酸胆碱和 1,2-二酰基甘油磷酸甘油具有磷脂酶 C 活性。它通过神经酰胺的产生介导参与应激诱导的细胞凋亡调节。
种属

Mouse
表达系统

Sf9 insect cells
标签

C-His
蛋白编号

Q04519 (M1-L626)
基因 ID

20597  [NCBI]
蛋白结构
N-term
SMPD1 (M1-L626)
Accession # Q04519
His
C-term
同用名
Sphingomyelin phosphodiesterase; Acid sphingomyelinase; ASMase; Smpd1; Asm
分子量

Approximately 63 kDa.
纯度

Greater than 85% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

SMPD1 Protein, Mouse (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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