USP14 蛋白, Human (N-His)

USP14, a proteasome-associated deubiquitinase, regulates ubiquitin dynamics by releasing it from proteins marked for degradation. As a reversible proteasome subunit, USP14 ensures ubiquitin replenishment. Beyond proteasomal protein turnover, it degrades CXCR4 for cell chemotaxis and inhibits ERAD under non-stressed conditions. USP14 also aids synaptic development at NMJs and stabilizes CGAS for antiviral defense, revealing its multifaceted role in cellular processes. USP14 Protein, Human (N-His) is the recombinant human-derived USP14 protein, expressed by E. coli, with N-6*His labeled tag. The total length of USP14 Protein, Human (N-His) is 494 a.a., with molecular weight of ~60 kDa.

USP14 是一种蛋白酶体相关的去泛素酶，在细胞过程中发挥着至关重要的作用，特别是在蛋白酶体泛素的动态调节中。作为蛋白酶体的可逆相关亚基，USP14 确保泛素化蛋白从目标降解中释放泛素，促进泛素在细胞环境中的再生。除了在蛋白酶体介导的蛋白质周转中发挥作用之外，USP14 在多种生理环境中也发挥着关键作用。它参与趋化因子受体 CXCR4 的降解，这是 CXCL12 诱导的细胞趋化性的关键事件。此外，USP14 在非应激条件下可作为内质网相关降解 (ERAD) 的生理抑制剂，与 ERN1 相互作用并调节未折叠内质网蛋白的降解。此外，USP14 有助于神经肌肉接头 (NMJ) 的突触发育和功能，并通过稳定病毒 DNA 传感器 CGAS 参与针对病毒的先天免疫防御，从而阻止其自噬降解。

Human

E. coli

N-6*His

P54578 (M1-Q494)

9097  [NCBI]

MPLYSVTVKWGKEKFEGVELNTDEPPMVFKAQLFALTGVQPARQKVMVKGGTLKDDDWGNIKIKNGMTLLMMGSADALPEEPSAKTVFVEDMTEEQLASAMELPCGLTNLGNTCYMNATVQCIRSVPELKDALKRYAGALRASGEMASAQYITAALRDLFDSMDKTSSSIPPIILLQFLHMAFPQFAEKGEQGQYLQQDANECWIQMMRVLQQKLEAIEDDSVKETDSSSASAATPSKKKSLIDQFFGVEFETTMKCTESEEEEVTKGKENQLQLSCFINQEVKYLFTGLKLRLQEEITKQSPTLQRNALYIKSSKISRLPAYLTIQMVRFFYKEKESVNAKVLKDVKFPLMLDMYELCTPELQEKMVSFRSKFKDLEDKKVNQQPNTSDKKSSPQKEVKYEPFSFADDIGSNNCGYYDLQAVLTHQGRSSSSGHYVSWVKRKQDEWIKFDDDKVSIVTPEDILRLSGGGDWHIAYVLLYGPRRVEIMEEESEQ

Approximately 60 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.