1. Immunology/Inflammation Apoptosis Autophagy
  2. Toll-like Receptor (TLR) TNF Receptor Interleukin Related Autophagy
  3. Resatorvid

Resatorvid  (Synonyms: 瑞沙托维; TAK-242; CLI-095)

目录号: HY-11109 纯度: 99.72%
COA 产品使用指南

Resatorvid (TAK-242) 是一种选择性的 TLR4 信号传导抑制剂。Resatorvid 抑制 NOTNF-αIL-6 的产生,其 IC50 值分别为 1.8 nM,1.9 nM,1.3 nM。Resatorvid 下调 TLR4 下游信号分子 MyD88 和 TRIF 的表达。Resatorvid 抑制自噬 (autophagy),并在各种炎症性疾病中起关键作用。

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Resatorvid Chemical Structure

Resatorvid Chemical Structure

CAS No. : 243984-11-4

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Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 349 篇科研文献

WB
IF
IHC
Proliferation Assay

    Resatorvid purchased from MCE. Usage Cited in: Metabolites. 2022, 12(11), 1132

    Resatorvid (TAK-242; 0.1, 1, 10, 50 µM; 24 h) inhibits the proliferation of B16 cells and A375 cells in a dose-dependent manner.

    Resatorvid purchased from MCE. Usage Cited in: Anesthesiology. 2019 Feb;130(2):292-308.   [Abstract]

    Detection of PCNA by immunohistochemistry with the treatment of 2DG, MET and COM.

    Resatorvid purchased from MCE. Usage Cited in: Anesthesiology. 2019 Feb;130(2):292-308.   [Abstract]

    The detection of cell cycle-related proteins by western blotting with the treatment of 2DG, MET and COM.

    Resatorvid purchased from MCE. Usage Cited in: Anesthesiology. 2019 Feb;130(2):292-308.   [Abstract]

    Detection of activated signaling molecules in the mTOR pathway via western blotting with the treatment of 2DG, MET and COM.

    Resatorvid purchased from MCE. Usage Cited in: Anesthesiology. 2019 Feb;130(2):292-308.   [Abstract]

    Detection of PCNA by western blotting with the treatment of 2DG, MET and COM.

    Resatorvid purchased from MCE. Usage Cited in: Anesthesiology. 2019 Feb;130(2):292-308.   [Abstract]

    Detection of the expression of key glucose metabolism enzymes via western blotting with the treatment of 2DG, MET and COM.

    Resatorvid purchased from MCE. Usage Cited in: Anesthesiology. 2019 Feb;130(2):292-308.   [Abstract]

    The detection of activated signaling molecules of the ERK pathway via western blotting with the treatment of 2DG, MET and COM.

    Resatorvid purchased from MCE. Usage Cited in: Chem Biol Interact. 2019 Feb 1;299:179-185.  [Abstract]

    Western blot analysis of TLR4 and MyD88 showing effect of TLR4 inhibitor (CLI-095) on PQ-induced inflammation in BV2 microglia. The blots are representative of three independent experiments. The bar diagrams which reflect mean fold change in the expression levels of TLR4 and MyD88 with respect to the control.

    Resatorvid purchased from MCE. Usage Cited in: FASEB J. 2019 Mar;33(3):3451-3464.  [Abstract]

    Western blot analyses of TLR4 and MAP4K4, and moesin phosphorylation in HRMECs with the different treatments of TAK-242 or LPS.

    Resatorvid purchased from MCE. Usage Cited in: FASEB J. 2019 Mar;33(3):3451-3464.  [Abstract]

    Western blot analyses of TLR4 and MAP4K4 in the retina of PN 17 OIR mice with or without TAK-242 treatment.

    Resatorvid purchased from MCE. Usage Cited in: Front Physiol. 2019 Mar 1;10:165.  [Abstract]

    Effect of EP and TAK-242 treatment on CD68 protein expressions in aortic sinus of CUMS ApoE-/- mice. Over a 16-week period treatment, entire aorta from the aortic root to the iliac bifurcation is harvested, and then frozen-sections of aortic sinus are performed for immunofluorescent staining of CD68. Representative images of CD68 expression in aortic sinus.

    Resatorvid purchased from MCE. Usage Cited in: EBioMedicine. 2018 Nov;37:78-90.  [Abstract]

    TAK-242 alters NF-κB downstream targets in PSAP overexpressing SNB19 and GSC-21 cells as measured by western blotting.

    Resatorvid purchased from MCE. Usage Cited in: Carbohyd Polym. 2018 Mar 1;183:207-218.  [Abstract]

    TAK-242 significantly reduces the protein expression of p-JNK, p-ERK, p-p38, p-Akt and LC3-II.

    Resatorvid purchased from MCE. Usage Cited in: J Neuroinflammation. 2018 Sep 4;15(1):252.  [Abstract]

    The phosphorylation of NF-κB p65 evoked by Mrp14 is decreased after TAK-242 treatment.

    Resatorvid purchased from MCE. Usage Cited in: Front Immunol. 2018 Jul 23;9:1667.  [Abstract]

    The inhibitory effect of TAK-242 on the TLR4 mRNA and protein expression is detected by RT-qPCR and Western Blot in NR8383 cells.

    Resatorvid purchased from MCE. Usage Cited in: Transplantation. 2018 Sep;102(9):1505-1513.  [Abstract]

    Whole-islet lysates are prepared from islets with (TAK) or without (CTRL) early TLR4 blockade immediately after isolation (0 hours) and at 6- and 24-hours postisolation. Lysates are immunoblotted for ERK1/2, P38, SAPK, and P65, and analyzed for relative activation (phospho:total) normalized to β-actin.

    Resatorvid purchased from MCE. Usage Cited in: Cancer Sci. 2018 Aug;109(8):2391-2400.  [Abstract]

    A549 cells pretreated with or without 5 μM TAK-242 and/or 10 μM PP1 (Src inhibitor) for 1 h are treated with 50 ng/mL Resistin for 15 min. Total protein is isolated and expression of phosphorylated (p-)Src, Src, p-EGFR, and EGFR is detected by Western blot

    Resatorvid purchased from MCE. Usage Cited in: Front Pharmacol. 2018 Jan 30;9:28.  [Abstract]

    Effects of TAK-242 on the protein expressions of TLR4, MyD88, TRAF6, p-NF-κB p65, and total NF-κB p65 induced by Aβ1-42 in hBMECs by Western blotting analysis. The bands of TLR4, MyD88, TRAF6, p-NF-κB p65, total NF-κB p65, and β-actin. β-actin is used as a loading control.

    Resatorvid purchased from MCE. Usage Cited in: Int Immunopharmacol. 2018 Aug;61:266-276.  [Abstract]

    The protein expressions of TLR4 and NF-κB p65 are measured by western blot.

    Resatorvid purchased from MCE. Usage Cited in: Sci Rep. 2018 Jan 24;8(1):1535.  [Abstract]

    Effect of TAK242 on MAPKs phosphorylation in HUVECs treated with 9t18:1 and 11t18:1. HUVECs are treated with TAK242 (0.5, 1, 1.5 μM) for 30 min and then cultured with 9t18:1 for 24 h.

    Resatorvid purchased from MCE. Usage Cited in: Cell Immunol. 2018 Jun;328:86-92.  [Abstract]

    Washed platelets are dealt with LPS (10 ug/mL, 30 min), LPS (10 ug/mL, 30 min) and TAK242 (132 nM, 30 min) or vehicle. PKC, AKT and p38 are detected by western blotting.

    Resatorvid purchased from MCE. Usage Cited in: Cancer Prev Res (Phila). 2018 May;11(5):265-278.  [Abstract]

    There is a remarkable reduction in average levels of p21waf1 expression in the Prevention tumors compared to controls.

    Resatorvid purchased from MCE. Usage Cited in: J Mol Neurosci. 2018 Dec;66(4):535-546.  [Abstract]

    The increasing of TRAM1 is reversed by TAK242.

    Resatorvid purchased from MCE. Usage Cited in: J Mol Neurosci. 2018 Dec;66(4):535-546.  [Abstract]

    In SC, p-NF-κB production is inhibited by TRAM1 siRNA or TAK242.

    Resatorvid purchased from MCE. Usage Cited in: J Mol Neurosci. 2018 Dec;66(4):535-546.  [Abstract]

    In DRG, p-NF-κB production is inhibited by TRAM1 siRNA or TAK242.

    Resatorvid purchased from MCE. Usage Cited in: Neurosci Lett. 2018 Nov 1;686:33-40.   [Abstract]

    Images show the expression of MCPIP1 after the treatment of of PBS and LPS (with TAK-242, C29, FPS-ZM1).

    Resatorvid purchased from MCE. Usage Cited in: Neurosci Lett. 2018 Nov 1;686:33-40.   [Abstract]

    Western Blot analyses show the protein expression of MCPIP1 after the treatment of PBS and LPS (with TAK-242, C29, FPS-ZM1).

    Resatorvid purchased from MCE. Usage Cited in: Cell Physiol Biochem. 2018;47(4):1497-1508.  [Abstract]

    TAK-242 inhibits coronary microembolization (CME)-induced TLR4 upregulation and NLRP3 inflammasome activation.

    Resatorvid purchased from MCE. Usage Cited in: Oxid Med Cell Longev. 2018 May 16;2018:6296802.  [Abstract]

    HK-2 cells are treated with TLR4 inhibitor (TAK242, 5 μM) for 2 h prior to HG (30 mM) treatment for 2 h and with NF-κB blocker (Parthenolide, 10 μM) and HG (30 mM) for 2 h, and the samples are collected for Western blot analysis.

    Resatorvid purchased from MCE. Usage Cited in: Front Immunol. 2017 Dec 13;8:1808.  [Abstract]

    The protein expression levels of Tgm2 in the 8-week Sj-infected mouse liver with TAK242 treatment are significantly reduced compared to those of the non-treated control.

    Resatorvid purchased from MCE. Usage Cited in: Life Sci. 2017 Feb 1;170:25-32.  [Abstract]

    Inhibition of TLR4 signaling attenuates PQ-induced NF-B activation and cytokine production. A549 cells are pre-treated 1 M TAK-242, followed by treatment with 300 M PQ for 24 h. The levels of (A) p-IB, (B) nuclear NF-B and (C) TLR4 are examined by Western blot analysis. (D) The relative mRNA expressions of TNF-, IL-1and IL-6 are measured by real-time PCR.

    Resatorvid purchased from MCE. Usage Cited in: Sci Rep. 2017 Mar 8;7:43834.  [Abstract]

    Compared with the expression in the DM + MCAO group, the expression of Bcl-2, Bax, cleaved caspase-3, TNF-α, IL-1β and TLR4 proteins is greatly reduced in the DM + MCAO + TAK242 group.

    Resatorvid purchased from MCE. Usage Cited in: PLoS One. 2017 May 24;12(5):e0178147.  [Abstract]

    (A) Titration of TAK-242 concentration. RPTC cells are cultured for 12 h in low glucose or high glucose medium with or without TAK-242. (B) MyD88 upregulation is also inhibited by TAK-242 under high glucose conditions by Western blotting.

    Resatorvid purchased from MCE. Usage Cited in: Mol Med Rep. 2017 Sep;16(3):3111-3116.  [Abstract]

    TLR4 inhibitor TAK 242 suppresses the TLR4/IRAK/NF ΚB signaling pathway in PASMCs. Western blot analysis is performed to detect the levels of p IRAK, IRAK, p IKK, IKK, p IκB, IκB and NF κB p65, and the representative blot results are shown.

    Resatorvid purchased from MCE. Usage Cited in: Graefes Arch Clin Exp Ophthalmol. 2017 Nov;255(11):2199-2210.  [Abstract]

    The effect of TAK-242 on the expression levels of NF-κB and MyD88 in retinal ganglion cells (RGCs) under high glucose are detected by Western blotting.

    Resatorvid purchased from MCE. Usage Cited in: Graefes Arch Clin Exp Ophthalmol. 2017 Nov;255(11):2199-2210.  [Abstract]

    The effect of TAK-242 on the expression levels of TRAF6 and NLRP3 in retinal ganglion cells (RGCs) under high glucose are detected by Western blotting.

    Resatorvid purchased from MCE. Usage Cited in: Chin Med J (Engl). 2017 Apr 20;130(8):906-913.  [Abstract]

    The levels of TLR4, IL-1β mRNA, and protein in TAK242 group are much lower than those in IgANTAK242 group.

    Resatorvid purchased from MCE. Usage Cited in: Oncotarget. 2017 May 9;8(19):31802-31814.  [Abstract]

    Activation of the β-catenin signaling pathway by Fn (F01) can be inhibited by both the TLR4 inhibitor (TAK-242) and PAK1 inhibitor (IPA-3).

    Resatorvid purchased from MCE. Usage Cited in: Cell Physiol Biochem. 2017;41(4):1675-1683.  [Abstract]

    p-IκBα protein expression detected by western blot.

    Resatorvid purchased from MCE. Usage Cited in: Cell Physiol Biochem. 2017;41(4):1675-1683.  [Abstract]

    Cleaved caspase-3 protein expression detected by western blot.

    Resatorvid purchased from MCE. Usage Cited in: Cell Physiol Biochem. 2017;41(4):1675-1683.  [Abstract]

    TLR4 protein expression detected by western blot.

    Resatorvid purchased from MCE. Usage Cited in: Cell Physiol Biochem. 2017;41(4):1675-1683.  [Abstract]

    NF-κB p65 (nuclei) protein expression detected by western blot.

    Resatorvid purchased from MCE. Usage Cited in: Cell Physiol Biochem. 2017;41(4):1675-1683.  [Abstract]

    MyD88 protein expression detected by western blot.

    Resatorvid purchased from MCE. Usage Cited in: J Neurochem. 2016 May;137(4):576-88.  [Abstract]

    TAK-242 markedly inhibits the up-regulation of IRAK-M and the down-regulation of p-IRAK-1 in rHMGB1-pretreated rats underwent MCAO. WB analysis of IRAK-M and p-IRAK-1 in rat ipsilateral hemispheres.

    Resatorvid purchased from MCE. Usage Cited in: Sci Rep. 2016 Aug 4;6:30957.  [Abstract]

    SEA promotes the senescence of LX-2 cells through activation of TLR4 signaling. Western blot analysis for P-p53 or TLR4 in LX-2 cells treated with SEA or TLR4 inhibitor TAK-242 (1 nM).

    Resatorvid purchased from MCE. Usage Cited in: Sci Rep. 2016 Jun 9;6:27866.  [Abstract]

    Examination of TLR4 expression level in each group. The protein and mRNA levels of TLR4 are down-regulated by pretreatment with TAK-242.

    Resatorvid purchased from MCE. Usage Cited in: Photochem Photobiol. 2016 Nov;92(6):816-825.  [Abstract]

    Western blot analysis reveals that Resatorvid-based inhibition of TLR4 is able to block UV-induced phosphorylation of MAP kinases (p38 and JNK) and Akt.

    Resatorvid purchased from MCE. Usage Cited in: Oncol Lett. 2016 Aug;12(2):1034-1040.  [Abstract]

    Involvement of TLR4 signaling pathway in HIF-1α silencing affects inflammatory and apoptosis under hypoxic conditions. In total, 30 mg total lysate is harvested with radioimmunoprecipitation assay buffer to detect TLR4, HIF-1α, caspase-3, MyD88, p-ASK1 and p-p38 protein expression by western blot analysis in human hepatocellular carcinoma HepG2 cells. The expression of TLR4, HIF-1α, MyD88, p-ASK1 NS and p-p38 in shHIF-1α transfected cells is significantly higher compared with the parental cells.

    Resatorvid purchased from MCE. Usage Cited in: Am J Physiol Gastrointest Liver Physiol. 2016 Dec 1;311(6):G1091-G1104.  [Abstract]

    Effects of TAK 242 and Catalpol on the expression of TLR-4 and activation of Src, PI3K, FAK, and Cathepsin B induced by LPS in HUVECs. Representative Western blots of TLR-4, p-Src, Src, p-PI3K, PI3K, p-FAK, FAK, activated cathepsin B, and cathepsin B proenzyme in different groups.

    Resatorvid purchased from MCE. Usage Cited in: PLoS One. 2015 Nov 10;10(11):e0142456.  [Abstract]

    Renal protein expression of E-cadherin (an epithelial marker), and Fibronectin and a-SMA (mesenchymal markers) are assayed in rats treated with Aldo-salt or with Aldo-salt plus TAK-242. TAK-242 reverses the Aldo-salt-induced renal epithelial-mesenchymal transition.

    查看 Toll-like Receptor (TLR) 亚型特异性产品:

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Resatorvid (TAK-242) is a selective Toll-like receptor 4 (TLR4) inhibitor. Resatorvid inhibits NO, TNF-α and IL-6 production with IC50s of 1.8 nM, 1.9 nM and 1.3 nM, respectively. Resatorvid downregulates expression of TLR4 downstream signaling molecules MyD88 and TRIF. Resatorvid inhibits autophagy and plays pivotal role in various inflammatory diseases[1][2].

    IC50 & Target[1][2]

    TLR4

     

    TNF-R

    1.9 nM (IC50)

    IL-6

    1.3 nM (IC50)

    体外研究
    (In Vitro)

    Resatorvid 抑制 LPS 刺激的人外周血单核细胞 (PBMC) 产生 NO、TNF-α 和 IL-6,IC50 值为 11 至 33 nM[1]
    Resatorvid (1-100 nM;4 小时) 抑制 RAW264.7 细胞中 LPS 和 IFN-γ 诱导的 IL-6 和 TNF-α 的 mRNA 表达[1]
    Resatorvid (1-100 nM;15 分钟;PBMCs 细胞) 显著抑制 LPS 诱导的细胞外信号调节激酶 1/2 (Erk1/2)、p38 和 JNK/SAPK 的磷酸化以及降解 IκBβ 浓度为 100 nM[1]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    RT-PCR[1]

    Cell Line: RAW264.7 cells
    Concentration: 1 nM, 10 nM, 100 nM
    Incubation Time: 4 hours
    Result: TNF-α and IL-6 mRNA expression levels were clearly suppressed at concentrations of 10 to 100 nM.

    Western Blot Analysis[1]

    Cell Line: PBMCs cells
    Concentration: 1 nM, 10 nM, 100 nM
    Incubation Time: 15 minutes
    Result: The phosphorylation of mitogen-activated protein kinases induced by LPS was also inhibited in a concentration-dependent manner.
    体内研究
    (In Vivo)

    Resatorvid (TAK-242;3 mg/kg;腹膜内注射;持续 2 天;雄性 C57BL/6 小鼠) 预处理可显著且显著地逆转 LPS 诱导的体重减轻、TA 肌肉损失和肌肉力量损失。TAK-242 逆转 LPS 引起的肌肉纤维收缩并增加间质空间。TAK-242 阻断 LPS 给药小鼠的全身分解代谢细胞因子释放和骨骼肌蛋白水解[3]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male C57BL/6 mice (8-12 weeks of age) treated with lipopolysaccharide (LPS)[3]
    Dosage: 3 mg/kg
    Administration: Intraperitoneal injection; for 2 days
    Result: Pretreatment of mice reduced or reversed all the detrimental effects of LPS.
    Clinical Trial
    分子量

    361.82

    Formula

    C15H17ClFNO4S

    CAS 号
    性状

    固体

    颜色

    Off-white to yellow

    中文名称

    瑞沙托维

    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    溶解性数据
    In Vitro: 

    DMSO 中的溶解度 : 100 mg/mL (276.38 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

    Ethanol 中的溶解度 : 20 mg/mL (55.28 mM; 超声加热助溶)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 2.7638 mL 13.8190 mL 27.6381 mL
    5 mM 0.5528 mL 2.7638 mL 5.5276 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    In Vivo:

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
    ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂: 5% DMSO    40% PEG300    5% Tween-80    50% Saline

      Solubility: 5.5 mg/mL (15.20 mM); 悬浊液; 超声助溶

    • 方案 二

      请依序添加每种溶剂: 5% DMSO    95% (20% SBE-β-CD in Saline)

      Solubility: 5.5 mg/mL (15.20 mM); 悬浊液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    请输入您的动物体内配方组成:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    如果您的动物是免疫缺陷鼠或者体弱鼠,建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
    方案所需 助溶剂 包括:DMSO ,均可在 MCE 网站选购。 Tween 80,均可在 MCE 网站选购。
    计算结果
    工作液所需浓度 : mg/mL
    储备液配制方法 : mg 药物溶于 μL  DMSO(母液浓度为 mg/mL)。
    您所需的储备液浓度超过该产品的实测溶解度,以下方案仅供参考,如有需要,请与 MCE 中国技术支持联系。
    动物实验体内工作液的配制方法 : 取 μL DMSO 储备液,加入 μL  μL ,混合均匀至澄清,再加 μL Tween 80,混合均匀至澄清,再加 μL 生理盐水
    连续给药周期超过半月以上,请谨慎选择该方案。
    请确保第一步储备液溶解至澄清状态,从左到右依次添加助溶剂。您可采用超声加热 (超声清洗仪,建议频次 20-40 kHz),涡旋吹打等方式辅助溶解。
    纯度 & 产品资料

    纯度: 99.95% ee.: 98.00%

    参考文献

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    Ethanol / DMSO 1 mM 2.7638 mL 13.8190 mL 27.6381 mL 69.0951 mL
    5 mM 0.5528 mL 2.7638 mL 5.5276 mL 13.8190 mL
    10 mM 0.2764 mL 1.3819 mL 2.7638 mL 6.9095 mL
    15 mM 0.1843 mL 0.9213 mL 1.8425 mL 4.6063 mL
    20 mM 0.1382 mL 0.6910 mL 1.3819 mL 3.4548 mL
    25 mM 0.1106 mL 0.5528 mL 1.1055 mL 2.7638 mL
    30 mM 0.0921 mL 0.4606 mL 0.9213 mL 2.3032 mL
    40 mM 0.0691 mL 0.3455 mL 0.6910 mL 1.7274 mL
    50 mM 0.0553 mL 0.2764 mL 0.5528 mL 1.3819 mL
    DMSO 60 mM 0.0461 mL 0.2303 mL 0.4606 mL 1.1516 mL
    80 mM 0.0345 mL 0.1727 mL 0.3455 mL 0.8637 mL
    100 mM 0.0276 mL 0.1382 mL 0.2764 mL 0.6910 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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