2. Academic Validation
  3. Evidence for expression and function of angiotensin II receptor type 1 in pulmonary epithelial cells

Evidence for expression and function of angiotensin II receptor type 1 in pulmonary epithelial cells

  • Respir Physiol Neurobiol. 2014 May 1;195:37-40. doi: 10.1016/j.resp.2014.02.001.
Omaima Ashry 1,  Anja Schnecko 2,  Wolfgang G Clauss 3,  Martin Fronius 4
Affiliations

Affiliations

  • 1 Institute of Animal Physiology, Justus-Liebig-University Giessen, Giessen, Germany. Electronic address: omaimashry@yahoo.com.
  • 2 Institute of Animal Physiology, Justus-Liebig-University Giessen, Giessen, Germany. Electronic address: anja.schnecko@physzool.bio.uni-giessen.de.
  • 3 Institute of Animal Physiology, Justus-Liebig-University Giessen, Giessen, Germany. Electronic address: wolfgang.g.clauss@physzool.bio.uni-giessen.de.
  • 4 Institute of Animal Physiology, Justus-Liebig-University Giessen, Giessen, Germany. Electronic address: martin.fronius@otago.ac.nz.
PMID: 24530803 DOI: 10.1016/j.resp.2014.02.001
Abstract

There is a growing evidence that the peptide hormone angiotensin II (ANGII) can act as an auto-/paracrine mediator to regulate epithelial ion transport processes. The present study focused on the impact of ANGII on transepithelial ion transport in pulmonary epithelia. Transcripts for the ANGII receptor type 1 (ATR1) were detected in lungs of Xenopus laevis and H441 cells (human pulmonary epithelial cell line). Native Xenopus lung preparations were used for Ussing chamber recordings and apically applied ANGII (10μM) induced a significant increase of short-circuit current (ISC: 8±2%, n=13). Pre-incubation with losartan (LOS), an antagonist of ATR1 prevented the effect of ANGII on ISC. Transcripts for ATR1 in Xenopus lungs and H441 cells were detected and an increase of ISC was observed by ANGII in native Xenopus lung epithelia. This indicates that ANGII is a potential auto-/paracrine mediator for ion transport regulation in pulmonary epithelia.

Keywords

Angiotensin II; Angiotensin II receptor; Pulmonary epithelium; Pulmonary ion transport; Ussing chamber; Xenopus laevis.

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