1. Academic Validation
  2. Platycodon grandifloras polysaccharides inhibit mitophagy injury induced by Cr (VI) in DF-1 cells

Platycodon grandifloras polysaccharides inhibit mitophagy injury induced by Cr (VI) in DF-1 cells

  • Ecotoxicol Environ Saf. 2020 Oct 1;202:110901. doi: 10.1016/j.ecoenv.2020.110901.
Jiajia Hao 1 Yafen Song 2 Bin Tian 3 Changxi Qi 1 Liping Li 1 Lumei Wang 4 Yuxiao Xing 1 Xiaona Zhao 5 Jianzhu Liu 6
Affiliations

Affiliations

  • 1 College of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong, 271018, China.
  • 2 Department of Veterinary Culture Collection, China Institute of Veterinary Drug Control, 8 Nandajie, Zhongguancun, Haidian District, Beijing, 100081, China.
  • 3 Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
  • 4 Research Center for Animal Disease Control Engineering, Shandong Agricultural University, Tai'an, Shandong, 271018, China.
  • 5 Research Center for Animal Disease Control Engineering, Shandong Agricultural University, Tai'an, Shandong, 271018, China. Electronic address: zhaoxn@sdau.edu.cn.
  • 6 College of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong, 271018, China. Electronic address: liujz@sdau.edu.cn.
Abstract

This study aimed to investigate the role of Platycodon grandiflorus polysaccharide (PGPS) in chromium (VI)-induced Autophagy in a chicken embryo fibroblast cell lines (DF-1 cells). DF-1 cells were exposed to Cr (VI), PGPSt, and Cr (VI) + PGPSt, and their effects on cell viability, Reactive Oxygen Species (ROS), mitochondrial membrane potential (MMP), and autophagy-related proteins were examined. The results showed that the cell viability was reduced after Cr (VI) treatment, and 3-MA, CsA or PGPSt suppressed this decrease. Cr (VI) treatment increased the ROS levels and decreased the MMP, thereby enhancing the expression of mitochondrial Autophagy marker proteins (PINK1, Parkin, and LC3-II), inhibiting mitophagy Autophagy protein TOMM20 expression, and promoting the degradation of autophagy-related marker p62. These changes led to exceeding mitochondrial Autophagy and cell trauma and could be mitigated by PGPSt. Overall, our research showed that Cr (VI) can induce exceeding mitochondrial Autophagy in DF-1 cells, whereas PGPSt can improve Cr (VI)-induced mitochondrial Autophagy by inhibiting ROS and restoring MMP.

Keywords

DF-1 cells; Mitophagy; Platycodon grandifloras polysaccharides; chromium(VI).

Figures
Products