1. Academic Validation
  2. The Acetylation Modification of SP1 Regulates the Protein Stability in Silkworm

The Acetylation Modification of SP1 Regulates the Protein Stability in Silkworm

  • Appl Biochem Biotechnol. 2022 Apr;194(4):1621-1635. doi: 10.1007/s12010-021-03757-9.
Zihan Sun  # 1 Yafei Ma  # 1 Yue Liu 2 Jiao Lv 1 Dan Wang 1 Zhengying You 1 Caiying Jiang 1 Qing Sheng 1 Zuoming Nie 3
Affiliations

Affiliations

  • 1 College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, China.
  • 2 Zhejiang Economic & Trade Polytechnic, Hangzhou, 310018, China.
  • 3 College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, China. nzm16@tsinghua.org.cn.
  • # Contributed equally.
Abstract

Acetylation is a highly conservative and reversible post-translational modification. Acetylation modification can regulate gene expression by altering protein function and is widely identified in an increasing number of species. Previously, the acetylated proteome of silkworm was identified by combining acetylated polypeptide enrichment with nano-HPLC/MS/MS; the identification revealed that the SP proteins (SPs) were high acetylated. In this study, the acetylation of SP1, one of the SPs, was further confirmed using immunoprecipitation (IP) and Western blotting. Then, we found the acetylation could upregulate SP1 protein expression by enhancing the protein stability. Further research found that the acetylation of SP1 protein can competitively inhibit its ubiquitination and thus improve the stability and cell accumulation of SP1 protein by inhibiting the ubiquitin-mediated Proteasome degradation pathway. This result provides a basis for acetylation to regulate the nutrient storage and utilization of silkworm.

Keywords

Acetylation; Protein stability; SP1; Ubiquitination.

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