1. Signaling Pathways
  2. Cell Cycle/DNA Damage
  3. CRISPR/Cas9

CRISPR/Cas9

CRISPR/Cas9 (Clustered Regularly-Interspaced Short Palindromic Repeats/Cas9) is a bacterial immune system that has been adapted for genome editing in mammalian cells. Cas9 is a programmable nuclease that generates double-stranded breaks (DSB) in DNA dictated by binding of a ~20 nucleotide recombinant “guide RNA” (gRNA) to the target site. DSB’s produced by Cas9 are most often repaired through the cell’s error-prone non-homologous end joining (NHEJ) pathway, resulting in small insertions or deletions (indels). The vast majority of indels shift the reading frame, introducing a premature stop codon or resulting in nonsense mediated decay of the mRNA-effectively “knocking out” a gene.

The CRISPR/Cas9 system has substantially advanced efforts in specific gene editing and has been successfully applied to modify the episomal genomes of human and other organisms. The CRISPR/Cas9 system utilizes a prokaryotic RNA-guided programmable nuclease that can make a double-strand DNA break (DSB) at a specific site under the guidance of a leading RNA. This DSB process depends on the co-expression of two basic components: a guide RNA (gRNA) and Cas9 nuclease. Making a specific DSB can trigger DNA repair via either error-prone non-homologous end joining (NHEJ) or homology-directed repair (HDR). In the presence of the CRISPR/Cas9 system, the NHEJ inhibitor SCR7 is proven to increase the efficiency of Cas9-mediated HDR by at least by 7-fold in mammalian cells. Genome editing via CRISPR/Cas9 has become an efficient and reliable way to make precise, targeted changes to the genome of living cells.

CRISPR/Cas9 相关产品 (9):

Cat. No. Product Name Effect Purity
  • HY-16592
    Brefeldin A Activator 99.79%
    Brefeldin A (BFA) 是一种内酯抗生素,是蛋白质运输的特定抑制剂。Brefeldin A 阻断分泌蛋白和膜蛋白从内质网向高尔基体的转运。Brefeldin A 也是一种自噬 (autophagy) 和 mitophagy 抑制剂。Brefeldin A 还是一种 CRISPR/Cas9 激动剂,可抑制 HSV-1病毒,并具有抗癌活性。
  • HY-13520
    Nocodazole Activator 98.68%
    Nocodazole是快速可逆的 microtubule 抑制剂。 Nocodazole与β-微管蛋白结合并破坏微管组装/拆卸动力学,从而防止有丝分裂并诱导肿瘤细胞凋亡。Nocodazole 抑制 Bcr-Abl,增强 CRISPR/Cas9 的活性。
  • HY-11006
    KU-57788 Activator 99.35%
    KU-57788 是一种有效的,选择性的 DNA-PK 抑制剂,IC50 值为 13 nM。
  • HY-12742
    SCR7 Agonist
    SCR7 是一种不稳定的形式,可以自动循环成​​稳定形式的 SCR7 pyrazine。SCR7 pyrazine 是一种 DNA 连接酶 IV (DNA ligase IV) 抑制剂,它以连接酶 IV 依赖的方式阻断非同源末端连接 (NHEJ)。SCR7 也是一种 CRISPR/Cas9 的增强子,可提高 Cas9 介导的同源性定向修复 (HDR) 的效率。SCR7 pyrazine 诱导细胞凋亡 (apoptosis) 并具有抗癌活性。
  • HY-17413
    Zidovudine Agonist 99.96%
    Zidovudine 是一种核苷逆转录酶抑制剂 (NRTI),广泛用于治疗 HIV感染。Zidovudine 增强 CRISPR/Cas9调节的编辑频率。
  • HY-136251
    BRD0539 Inhibitor
    BRD0539 是一种可渗透细胞的,无毒的 CRISPR-Cas9 抑制剂。在体外 DNA 裂解试验中,BRD0539 可抑制化脓性链球菌 Cas9 (SpCas9),IC50 为 22 μM。
  • HY-107845
    SCR7 pyrazine Agonist 99.75%
    SCR7 pyrazine 是一种 DNA 连接酶 IV (DNA ligase IV) 抑制剂,它以连接酶 IV 依赖的方式阻断非同源末端连接 (NHEJ)。SCR7 pyrazine 也是一种 CRISPR/Cas9 的增强子,可提高 Cas9 介导的同源性定向修复 (HDR) 的效率。SCR7 pyrazine 诱导细胞凋亡 (apoptosis) 并具有抗癌活性。
  • HY-19793
    RS-1 Activator 99.55%
    RS-1 是一种 RAD51 的激活剂,同时可增强 CRISPR/Cas9 的活性。
  • HY-19334
    L755507 Agonist >98.0%
    L755507 是一个有效的, 选择性强的 β3-AR 激动剂, 其 IC50 值为 35 nM。
Isoform Specific Products

Your Search Returned No Results.

Sorry. There is currently no product that acts on isoform together.

Please try each isoform separately.