1. Signaling Pathways
  2. Cell Cycle/DNA Damage
  3. CRISPR/Cas9

CRISPR/Cas9 

The CRISPR/Cas9 system derived from bacterial adaptive immune systems is one of the most powerful genome editing technology. It is an RNA-guided genome editing tool that consists of a Cas9 nuclease and a single-guide RNA (sgRNA). By base-pairing with a DNA target sequence, the sgRNA enables Cas9 to recognize and cut a specific target DNA sequence, generating double strand breaks (DSBs) that trigger cell repair mechanisms and mutations at or near the DSBs sites. CRISPR/Cas9 technology has been studied extensively and its application has been expanded from the modification of the gene in cells to organisms. The potential role of CRISPR/Cas9 in gene therapy has made it to become one of the hottest pots in cancer treatment. Different concepts of CRISPR/Cas9-mediated cancer therapy, including tumor-related genes manipulating, tumor immunotherapy, tumor research modelling and anti-cancer drug resistance overcoming are established in various cancer types.

The greatest advantages of the CRISPR-Cas9 system are its simplicity and wide applicability in genome manipulations of almost all biological systems tested to date, including cell lines, stem cells, yeasts, worms, insects, rodents, and mammals. For a targetable DNA site, only a corresponding 20 nucleotide gRNA is needed to guide the CRISPR-Cas9 to cut the target DNA at the desired location. The repair of the broken DNA ends occurs either through NHEJ to generate indels, which has been used to generate random genomic mutations or through HDR in the presence of donor oligonucleotides or DNA fragments containing homologous sequences flanking the DSB sites to generate precise site-directed nucleotide or large gene replacements, leading to generation of targeted gene mutations or corrections.

CRISPR/Cas9 相关产品 (10):

目录号 产品名 作用方式 纯度
  • HY-16592
    Brefeldin A Activator
    Brefeldin A (BFA) 是一种内酯抗生素,是蛋白质运输的特定抑制剂。Brefeldin A 阻断分泌蛋白和膜蛋白从内质网向高尔基体的转运。Brefeldin A 也是一种自噬 (autophagy) 和 mitophagy 抑制剂。Brefeldin A 还是一种 CRISPR/Cas9 激动剂,可抑制 HSV-1病毒,并具有抗癌活性。
  • HY-13520
    Nocodazole Activator 98.63%
    Nocodazole (Oncodazole) 是快速可逆的 microtubule 抑制剂。 Nocodazole与β-微管蛋白结合并破坏微管组装/拆卸动力学,从而防止有丝分裂并诱导肿瘤细胞凋亡。Nocodazole 抑制 Bcr-Abl,增强 CRISPR/Cas9 的活性。
  • HY-11006
    KU-57788 Activator 99.35%
    KU-57788 (NU7441) 是一种高效的选择性 DNA-PK 抑制剂,IC50 为 14 nM。 KU-57788 是 NHEJ 通路抑制剂。KU-57788 还抑制 PI3K 和 mTOR,IC50 分别为 5.0 和 1.7 μM。
  • HY-17413
    Zidovudine Agonist 99.82%
    Zidovudine 是一种核苷逆转录酶抑制剂 (NRTI),有潜力用于 HIV 感染的研究。Zidovudine 增强 CRISPR/Cas9调节的编辑频率。
  • HY-107845
    SCR7 pyrazine Agonist 98.70%
    SCR7 pyrazine 是一种 DNA 连接酶 IV (DNA ligase IV) 抑制剂,它以连接酶 IV 依赖的方式阻断非同源末端连接 (NHEJ)。SCR7 pyrazine 也是一种 CRISPR/Cas9 的增强子,可提高 Cas9 介导的同源性定向修复 (HDR) 的效率。SCR7 pyrazine 诱导细胞凋亡 (apoptosis) 并具有抗癌活性。
  • HY-19793
    RS-1 Activator 99.55%
    RS-1 是一种 RAD51 的激活剂,同时可增强 CRISPR/Cas9 的活性。
  • HY-12742
    SCR7 Agonist 98.22%
    SCR7 是一种不稳定的形式,可以自动循环成​​稳定形式的 SCR7 pyrazine。SCR7 pyrazine 是一种 DNA 连接酶 IV (DNA ligase IV) 抑制剂,它以连接酶 IV 依赖的方式阻断非同源末端连接 (NHEJ)。SCR7 也是一种 CRISPR/Cas9 的增强子,可提高 Cas9 介导的同源性定向修复 (HDR) 的效率。SCR7 pyrazine 诱导细胞凋亡 (apoptosis) 并具有抗癌活性。
  • HY-19334
    L755507 Agonist ≥98.0%
    L755507 是一个有效的, 选择性强的 β3-AR 激动剂, 其 IC50 值为 35 nM。
  • HY-136572
    CEM114
    CEM114 是一种有效的化学表观遗传修饰剂 (CEM),可通过 CRISPR-Cas9 系统募集内源性染色质机制。
  • HY-136251
    BRD0539 Inhibitor
    BRD0539 是一种可渗透细胞的,无毒的 CRISPR-Cas9 抑制剂。在体外 DNA 裂解试验中,BRD0539 可抑制化脓性链球菌 Cas9 (SpCas9),IC50 为 22 μM。
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