1. Biology Dictionary
  2. CRISPR-Cas9

CRISPR-Cas9

释义:

CRISPR-Cas9 is an efficient, fast, easy technique for achieving knock-out gene in the cell. The mechanism has three steps: recognition, cleavage, and repair. The designed sgRNA directs Cas-9 and recognizes the target sequence. The Cas-9 nuclease is activated and makes double-stranded breaks (DSBs) when sgRNA recognizes the target sequence.

CRISPR-Cas9 是一种高效、快速、简便的细胞基因敲除技术。其机制分为三个步骤:识别、切割和修复。所设计的向导 RNA (sgRNA) 可引导 Cas-9 并识别目标序列。当 sgRNA 识别目标序列后,Cas-9 核酸酶会被激活并产生双链断裂 (DSB)。

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