2. Academic Validation
  3. Substrate-dependent activation of thermolysin by salt

Substrate-dependent activation of thermolysin by salt

  • Biosci Biotechnol Biochem. 2004 Aug;68(8):1811-3. doi: 10.1271/bbb.68.1811.
Hiroshi Oneda 1,  Yuko Muta,  Kuniyo Inouye
Affiliations

Affiliation

  • 1 Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
PMID: 15322372 DOI: 10.1271/bbb.68.1811
Abstract

Salt-activation of thermolysin was examined using a positively charged fluorescent substrate, (7-methoxycoumarin-4-yl)acetyl-L-Pro-L-Leu-Gly-L-Leu-[N(3)-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH(2) [MOCAc-PLGL(Dpa)AR]. Thermolysin activity increased in a biphasic exponential fashion and was 40 times higher in the presence of 4 M NaCl than in its absence. The degree of activation at X M NaCl was expressed as 4.7(x) when [NaCl](o) < 0.5 M and 2.3(x) when [NaCl](o) > 0.5 M respectively.

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