2. Academic Validation
  3. The spin trapping of superoxide with M4PO (3,3,5,5-tetramethylpyrroline-N-oxide)

The spin trapping of superoxide with M4PO (3,3,5,5-tetramethylpyrroline-N-oxide)

  • Free Radic Biol Med. 1990;8(1):57-60. doi: 10.1016/0891-5849(90)90145-9.
G R Buettner 1,  B E Britigan
Affiliations

Affiliation

  • 1 ESR Center, University of Iowa, Iowa City 52242.
PMID: 2157635 DOI: 10.1016/0891-5849(90)90145-9
Abstract

The spin trap 3,3,5,5-tetramethylpyrroline-N-oxide (M4PO) reacts with superoxide to produce a short-lived spin adduct (M4PO/.OOH, AN = 14.0 G, AH = 6.5 G) that decays by a first-order process (t1/2 approximately 35 s at pH 7.4). This short lifetime may limit its usefulness in studies of superoxide.

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