2. Academic Validation
  3. Chromatographic methods for characterization of poly(ethylene glycol)-modified polyamidoamine dendrimers

Chromatographic methods for characterization of poly(ethylene glycol)-modified polyamidoamine dendrimers

  • Anal Biochem. 2014 Mar 15:449:42-4. doi: 10.1016/j.ab.2013.12.011.
Eun Ji Park 1,  Hoon Cho 2,  Si Wouk Kim 3,  Dong Hee Na 4
Affiliations

Affiliations

  • 1 College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Republic of Korea.
  • 2 Department of Polymer Science and Engineering, Chosun University, Gwangju 501-759, Republic of Korea.
  • 3 Department of Environmental Engineering, Chosun University, Gwangju 501-759, Republic of Korea.
  • 4 College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Republic of Korea. Electronic address: dhna@knu.ac.kr.
PMID: 24361709 DOI: 10.1016/j.ab.2013.12.011
Abstract

The objective of this study was to develop chromatographic methods for the determination of the modification degree and the characterization of poly(ethylene glycol)-modified polyamidoamine dendrimers (PEG-PAMAMs). The PEG-PAMAMs were prepared by reacting PAMAM generation 4 with monomethoxy PEG-nitrophenyl carbonate (mPEG-NPC). The modification degrees of PEG-PAMAMs were determined by quantifying 4-nitrophenol released from mPEG-NPC after PEGylation reaction using high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The PEG-PAMAMs, which have poor UV absorbances, were characterized by HPLC with charged aerosol detection. This study demonstrates that the combination of these two detectors is a powerful tool for the preparation and characterization of PEG-PAMAMs.

Keywords

Charged aerosol detection; Chromatographic method; PEGylation; Polyamidoamine dendrimers.

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