1. Academic Validation
  2. The beneficial effects of polyethylene glycol-superoxide dismutase on ovarian tissue culture and transplantation

The beneficial effects of polyethylene glycol-superoxide dismutase on ovarian tissue culture and transplantation

  • J Assist Reprod Genet. 2015 Oct;32(10):1561-9. doi: 10.1007/s10815-015-0537-8.
Eun Jung Kim 1 2 Hee Jun Lee 1 Jaewang Lee 1 2 Hye Won Youm 1 Jung Ryeol Lee 3 4 Chang Suk Suh 1 2 Seok Hyun Kim 2
Affiliations

Affiliations

  • 1 Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, 82, Gumi-ro 173beon-gil, Bundang-gu, Seongnam, 463-707, Gyeonggi-do, South Korea.
  • 2 Department of Obstetrics and Gynecology, Seoul National University College of Medicine, 101, Daehak-ro, Jongno-gu, Seoul, 110-744, South Korea.
  • 3 Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, 82, Gumi-ro 173beon-gil, Bundang-gu, Seongnam, 463-707, Gyeonggi-do, South Korea. leejrmd@snu.ac.kr.
  • 4 Department of Obstetrics and Gynecology, Seoul National University College of Medicine, 101, Daehak-ro, Jongno-gu, Seoul, 110-744, South Korea. leejrmd@snu.ac.kr.
Abstract

Purpose: Reducing the ischemic damage from free radicals that is inflicted on ovarian tissue is critical for successful ovarian tissue transplantation. Polyethylene glycol-superoxide dismutase (PEG-SOD) is mimetic of superoxide dismutase (SOD) and powerful free radical scavenger acts by reducing superoxide anions. The objective of study was to evaluate effects of PEG-SOD on mouse ovarian tissues in in vitro culture and in autotransplantation.

Methods: Ovaries were collected and randomly divided into four groups that received different doses of PEG-SOD. To assess effects of PEG-SOD on in vitro cultures, four different doses of PEG-SOD were applied to in vitro culture media during in vitro culturing following ovarian tissue vitrification and warming. To evaluate effects of PEG-SOD on ovarian tissue transplantation, four different doses of PEG-SOD were applied for 2, 7, and 21 days to mice following vitrified-warmed mouse ovarian tissue autotransplantation.

Results: The percentage of primordial follicles was maintained at the highest dose of PEG-SOD for 2 h in vitro, and there was a significant decrease in the percentage of apoptotic follicles at 2 h, but not at later time points. The highest dose of PEG-SOD also maintained primordial, primary, and secondary follicles 2 days post-transplantation, but only primordial follicles were maintained up to 21 days after transplantation.

Conclusions: PEG-SOD is protective mainly toward primordial follicles only for a short interval in vitro, presumably via antioxidant effects. PEG-SOD may be a promising additive for preserving ovarian tissue integrity, at least for primordial follicles, up to 21 days post-transplantation.

Keywords

Fertility preservation; Ovarian tissue cryopreservation; Ovarian tissue transplantation; Polyethylene glycol-superoxide dismutase (PEG-SOD).

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