2. Academic Validation
  3. Cochliomycin A inhibits the larval settlement of Amphibalanus amphitrite by activating the NO/cGMP pathway

Cochliomycin A inhibits the larval settlement of Amphibalanus amphitrite by activating the NO/cGMP pathway

  • Biofouling. 2016;32(1):35-44. doi: 10.1080/08927014.2015.1121245.
Kai-Ling Wang 1 2 3,  Gen Zhang 4,  Jin Sun 5,  Ying Xu 2 3,  Zhuang Han 3 6,  Ling-Li Liu 4,  Chang-Lun Shao 1,  Qing-Ai Liu 1,  Chang-Yun Wang 1,  Pei-Yuan Qian 4 3
Affiliations

Affiliations

  • 1 a Key Laboratory of Marine Drugs, The Ministry of Education of China, School of Medicine and Pharmacy , Ocean University of China , Qingdao , PR China.
  • 2 b College of Life Science , Shenzhen University , Shenzhen , PR China.
  • 3 e Division of Life Science , Hong Kong University of Science and Technology, Clear Water Bay , Hong Kong SAR , PR China.
  • 4 c Environmental Science Program, School of Science , Hong Kong University of Science and Technology, Clear Water Bay , Hong Kong SAR , PR China.
  • 5 d Department of Biology , Hong Kong Baptist University , Hong Kong SAR , PR China.
  • 6 f Sanya Institute of Deep-sea Science and Engineering , Chinese Academy of Sciences , Sanya , PR China.
PMID: 26732984 DOI: 10.1080/08927014.2015.1121245
Abstract

Cochliomycin A is a compound with anti-barnacle settlement activity and low toxicity, but the molecular mechanism of the compound is unknown. Here, isobaric tags for the relative or absolute quantitation (iTRAQ) labeling proteomic method were applied to analyze changes in the proteome of Amphibalanus (=Balanus) amphitrite cyprids in response to cochliomycin A treatment. Cochliomycin A affected the Cytochrome P450, Glutathione S-transferase (GST) and NO/cGMP pathways, among which the NO/cGMP pathway was considered to play a key role in barnacle larval settlement, while the Cytochrome P450 and the GST pathways are mainly for detoxification. The results of Real-Time PCR further suggested the NO/cGMP pathway was activated in response to cochliomycin A. Larval settlement assays revealed that S-methylisothiourea sulfate (SMIS) and 1H-(1,2,4)oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) rescued cyprids from cochliomycin A-induced inhibition of larval settlement. The findings supported the hypothesis that cochliomycin A inhibited barnacle larval settlement by stimulating the NO/cGMP pathway.

Keywords

Cochliomycin A; antifouling; barnacle; cGMP; mechanism; nitric oxide.

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