1. Academic Validation
  2. An ultrafast turn-on thiol probe for protein labeling and bioimaging

An ultrafast turn-on thiol probe for protein labeling and bioimaging

  • Analyst. 2016 Mar 21;141(6):2009-15. doi: 10.1039/c5an02347e.
Jinyu Sun 1 Liangwei Zhang 1 Xiaolong Zhang 1 Yuesong Hu 1 Chunpo Ge 1 Jianguo Fang 1
Affiliations

Affiliation

  • 1 State Key Lab of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, PR China. fangjg@lzu.edu.cn.
Abstract

A novel turn-on type of ultrafast biothiol fluorescent probe, Naph-EA-mal, was designed, synthesized and evaluated. The probe contains a naphthalimide moiety as a fluorophore, a maleimide unit as a thiol acceptor, and 1,2-ethylenediamine as a linker. Naph-EA-mal displays high selectivity and a fast response toward thiols in aqueous solution. The reaction mechanism of the probe with thiols was confirmed by 1H NMR and HRMS. Test strips were fabricated and a sharp color change was observed by the naked-eye. Furthermore, Naph-EA-mal was successfully applied to label protein thiols, image thiols in living cells, quantify thiol content in cells lysate, and determine the reversible protein thiols oxidation in fixed cells.

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