2. Academic Validation
  3. Enzymatic Process for High-Yield Turanose Production and Its Potential Property as an Adipogenesis Regulator

Enzymatic Process for High-Yield Turanose Production and Its Potential Property as an Adipogenesis Regulator

  • J Agric Food Chem. 2016 Jun 15;64(23):4758-64. doi: 10.1021/acs.jafc.5b05849.
Min-Oh Park 1,  Byung-Hoo Lee 2,  Eunjin Lim 3,  Ji Ye Lim 3,  Yuri Kim 3,  Cheon-Seok Park 4,  Hyeon Gyu Lee 5,  Hee-Kwon Kang 1,  Sang-Ho Yoo 1
Affiliations

Affiliations

  • 1 Department of Food Science and Technology, and Carbohydrate Bioproduct Research Center, Sejong University , Gunja-Dong, Gwangjin-Gu, Seoul 143-747, Republic of Korea.
  • 2 Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University , Seongnam, Gyeonggi-do 461-701, Republic of Korea.
  • 3 Department of Nutritional Science and Food Management, Ewha Womans University , Seoul 120-750, Republic of Korea.
  • 4 Graduate School of Biotechnology, and Institute of Life Science and Resources, Kyung Hee University , Seocheon, Kiheung, Yongin 446-701, Republic of Korea.
  • 5 Department of Food and Nutrition, Hanyang University , 17 Haengdang-dong, Seongdong-gu, Seoul 133-791, Republic of Korea.
PMID: 27253611 DOI: 10.1021/acs.jafc.5b05849
Abstract

Turanose is a sucrose isomer naturally existing in honey and a promising functional sweetener due to its low glycemic response. In this study, the extrinsic fructose effect on turanose productivity was examined in Neisseria amylosucrase reaction. Turanose was produced, by increasing the amount of extrinsic fructose as a reaction modulator, with high concentration of sucrose substrate, which resulted in 73.7% of production yield. In physiological functionality test, lipid accumulation in 3T3-L1 preadipocytes in the presence of high amounts of pure glucose was attenuated by turanose substitution in a dose-dependent manner. Turanose treatments at concentrations representing 50%, 75%, and 100% of total glucose concentration in cell media significantly reduced lipid accumulation by 18%, 35%, and 72%, respectively, as compared to controls. This result suggested that turanose had a positive role in controlling adipogenesis, and enzymatic process of turanose production has a potential to develop a functional food ingredient for controlling obesity and related chronic diseases.

Keywords

Neisseria polysaccharea; adipogenesis; amylosucrase; fructose; turanose.

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