2. Academic Validation
  3. Biophysical study of resin acid effects on phospholipid membrane structure and properties

Biophysical study of resin acid effects on phospholipid membrane structure and properties

  • Biochim Biophys Acta. 2016 Nov;1858(11):2827-2838. doi: 10.1016/j.bbamem.2016.08.008.
Vivien Jagalski 1,  Robert Barker 2,  Daniel Topgaard 3,  Thomas Günther-Pomorski 4,  Björn Hamberger 4,  Marité Cárdenas 5
Affiliations

Affiliations

  • 1 Nano Science Center and Department of Chemistry, University of Copenhagen, Copenhagen, Denmark.
  • 2 Institute Laue Langevin, 71 avenue de Matyrs, CS, 20156, 38042 Grenoble Cedex 9, France.
  • 3 Division of Physical Chemistry, Department of Chemistry, Lund University, Sweden.
  • 4 Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark.
  • 5 Nano Science Center and Department of Chemistry, University of Copenhagen, Copenhagen, Denmark; Biofilm - Research Center for Biointerfaces and Department of Biomedical Science, Malmö University, Malmö, Sweden. Electronic address: cardenas@nano.ku.dk.
PMID: 27544924 DOI: 10.1016/j.bbamem.2016.08.008
Abstract

Hydrophobic resin acids (Ras) are synthesized by conifer trees as part of their defense mechanisms. One of the functions of Ras in plant defense is suggested to be the perturbation of the cellular membrane. However, there is a vast diversity of chemical structures within this class of molecules, and there are no clear correlations to the molecular mechanisms behind the RA's toxicity. In this study we unravel the molecular interactions of the three closely related Ras dehydroabietic acid, neoabietic acid, and the synthetic analogue dichlorodehydroabietic acid with dipalmitoylphosphatidylcholine (DPPC) model membranes and the polar lipid extract of soybeans. The complementarity of the biophysical techniques used (NMR, DLS, NR, DSC, Cryo-TEM) allowed correlating changes at the vesicle level with changes at the molecular level and the co-localization of Ras within DPPC monolayer. Effects on DPPC membranes are correlated with the physical chemical properties of the RA and their toxicity.

Keywords

DPPC; Lipid; Neutron reflectometry; Resin acids; Toxicity; ssNMR.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z