1. Academic Validation
  2. IL-27 regulates the adherence, proliferation, and migration of MSCs and enhances their regulatory effects on Th1 and Th2 subset generations

IL-27 regulates the adherence, proliferation, and migration of MSCs and enhances their regulatory effects on Th1 and Th2 subset generations

  • Immunol Res. 2017 Aug;65(4):903-912. doi: 10.1007/s12026-017-8929-8.
Fenghuang Xu 1 Junzhu Yi 1 Zhuoya Wang 1 Yejia Hu 2 Chunlei Han 3 Qun Xue 4 Xueguang Zhang 4 Xiying Luan 5 6
Affiliations

Affiliations

  • 1 Department of Immunology, Binzhou Medical University, Yantai, Shandong Province, 264003, People's Republic of China.
  • 2 Department of Pathophysiology, Binzhou Medical University, Yantai, Shandong Province, 264003, People's Republic of China.
  • 3 Department of Health Statistics, Binzhou Medical University, Yantai, Shandong Province, 264003, People's Republic of China.
  • 4 Medical College of Soochow University, Suzhou, Jiangsu, 215006, People's Republic of China.
  • 5 Department of Immunology, Binzhou Medical University, Yantai, Shandong Province, 264003, People's Republic of China. xyluan@sohu.com.
  • 6 Taishan Scholar Immunology Program, Binzhou Medical University, Yantai, Shandong Province, 264003, People's Republic of China. xyluan@sohu.com.
Abstract

Interleukin 27 (IL-27) regulates T cell function and is involved in inflammation. It has been reported that human placenta-derived mesenchymal stromal cells (hPMSCs) can inhibit T cell responses and attenuate inflammation reactions. However, it is unclear whether IL-27 can regulate hPMSC function. Here, we examined the effects of IL-27 upon adherence, migration, and proliferation as well as the immunomodulatory effects of hPMSCs. The results show that IL-27 Receptor α chain (IL-27Rα) is expressed in hPMSCs. IL-27 at 30 ng/ml inhibited hPMSC adherence and proliferation, while the migration of hPMSCs was promoted with IL-27 at doses of 20 or 30 ng/ml, as determined with use of real-time Cell Analysis (RTCA). Moreover, IL-27 promoted regulatory effects of hPMSCs through enhancing Th2 and suppressing Th1 subset generation from activated T cells in human peripheral blood. IL-27 also enhanced the ability of hPMSCs to secrete IL-10 from CD4+T cells through increased expression levels of the programmed death ligand 1 (PDL1) in hPMSCs via the Janus kinase (JAK)/signal transducer and activator of transcription 1 (STAT1) signaling pathway. In conclusion, IL-27 has significant modulatory effects on adherence, proliferation, and migration of hPMSCs. IL-27 increased PDL1 expression levels in hPMSCs via the JAK/STAT1 pathway, which then enhanced the regulatory effects of hPMSCs upon Th1 and Th2 cell generations and IL-10 secretion from CD4+T cells.

Keywords

IL-10; IL-27; Mesenchymal stromal cells (MSCs); Programmed death ligand 1 (PDL1); Th1; Th2.

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