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  3. Scriptaid cause histone deacetylase inhibition and cell cycle arrest in HeLa cancer cells: A study on structural and functional aspects

Scriptaid cause histone deacetylase inhibition and cell cycle arrest in HeLa cancer cells: A study on structural and functional aspects

  • Gene. 2017 Sep 5;627:379-386. doi: 10.1016/j.gene.2017.06.031.
M Janaki Ramaiah 1,  Shaik Mohammad Naushad 2,  A Lavanya 3,  Chatla Srinivas 3,  Tangutur Anjana Devi 3,  Sowganthika Sampathkumar 4,  Dhepikha Bharani Dharan 4,  Manika Pal Bhadra 5
Affiliations

Affiliations

  • 1 School of Chemical and Biotechnology, SASTRA University, Thanjavur 613401, India. Electronic address: janakiramaiah@scbt.sastra.edu.
  • 2 School of Chemical and Biotechnology, SASTRA University, Thanjavur 613401, India; Sandor Life Sciences Pvt. Ltd, Banjara Hills, Road No: 3, Hyderabad-500034, India.
  • 3 Chemical Biology Department, Indian Institute of Chemical Technology (IICT), Hyderabad 500007, India.
  • 4 School of Chemical and Biotechnology, SASTRA University, Thanjavur 613401, India.
  • 5 Chemical Biology Department, Indian Institute of Chemical Technology (IICT), Hyderabad 500007, India. Electronic address: manika@iict.res.in.
PMID: 28668345 DOI: 10.1016/j.gene.2017.06.031
Abstract

Scriptaid (SCR), a well-known histone deacetylase inhibitor, cause various cellular effects such as cell growth inhibition and Apoptosis. In this study, we have evaluated the anti-cancer effects of Scriptaid in HeLa cells, IMR-32 and HepG2 cells. Scriptaid inhibited the growth of HeLa cells with IC50 of 2μM at 48h in a dose-dependent manner. Flow-cytometric analysis indicated that SCR induced Apoptosis. Scriptaid was found to inhibit HDAC-8 effectively than other HDAC Inhibitor such as TSA as observed by HDAC-8 assay, Western blotting and modelling study. This observation was further strengthened by an artificial neuronal network (ANN) model.

Keywords

ANN; HDAC; HeLa; Scriptaid.

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