Anti-epileptic Effects of Valepotriate Isolated from Valeriana jatamansi Jones and Its Possible Mechanisms

Objective: This work aimed to investigate the anti-epileptic effects of valepotriate isolated from Valeriana jatamansi Jones and studied its possible mechanisms.

Methods: The anti-epileptic effects of valepotriate were studied using maximal electroshock-induced seizure (MES), pentylenetetrazole (PTZ)-induced epilepsy, and pentobarbital sodium-induced sleeping model in mice. The possible anti-epileptic mechanisms of valepotriate were investigated by analyzing the expressions of GABA_A, GABA_B, glutamic acid decarboxylase (GAD65), Bcl-2, and Caspase-3 in the brain using Western blot assay.

Results: The results indicated that valepotriate showed significant anti-epileptic activity against MES- and PTZ-induced epilepsy at doses of 5, 10, and 20 mg/kg, and ED₅₀ values for MES- and PTZ-induced epilepsy were 7.84 and 7.19 mg/kg, respectively. Furthermore, valepotriate (10 and 20 mg/kg) can significantly prolong sleeping time and shorten the latency time on the pentobarbital sodium-induced sleeping time test. Furthermore, valepotriate (5, 10, and 20 mg/kg) could significantly up-regulate the expression of GABA_A, GAD65, and Bcl-2 and down-regulate the expression of Caspase-3, but had no significant effect on the expression of GABA_B.

Conclusion: The results indicated that valepotriate had anti-epileptic activity and the mechanisms might be associated with regulation of GABA and inhibition of neuronal Apoptosis.

Summary: Anti-epileptic effect of valepotriate was investigated for the 1^st timeValepotriate showed notable anti-epileptic activityValepotriate can significantly increase the expression of GABA_A, glutamic acid decarboxylase 65, and Bcl-2 and reduce the expression of Caspase-3.