1. Academic Validation
  2. (-)-α-bisabolol prevents neuronal damage and memory deficits through reduction of proinflammatory markers induced by permanent focal cerebral ischemia in mice

(-)-α-bisabolol prevents neuronal damage and memory deficits through reduction of proinflammatory markers induced by permanent focal cerebral ischemia in mice

  • Eur J Pharmacol. 2019 Jan 5;842:270-280. doi: 10.1016/j.ejphar.2018.09.036.
Mara Yone Dias Fernandes 1 Marta Regina Santos do Carmo 2 Analu Aragão Fonteles 3 Julliana Catharina de Sousa Neves 4 Ana Thaís Araújo da Silva 5 Juliana Fernandes Pereira 6 Emerson de Oliveira Ferreira 7 Neila Maria Rocha de Lima 8 Kelly Rose Tavares Neves 9 Geanne Matos de Andrade 10
Affiliations

Affiliations

  • 1 Post-Graduate Programme in Pharmacology, Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Brazil. Electronic address: maraiony@hotmail.com.
  • 2 Post-Graduate Programme in Pharmacology, Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Brazil. Electronic address: martacarmo@yahoo.com.br.
  • 3 Post-Graduate Programme in Pharmacology, Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Brazil. Electronic address: analufont@hotmail.com.
  • 4 Post-Graduate Programme in Pharmacology, Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Brazil. Electronic address: jucatharina@yahoo.com.br.
  • 5 Post-Graduate Programme in Pharmacology, Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Brazil. Electronic address: silvaata@yahoo.com.
  • 6 Post-Graduate Programme in Medical Sciences, Department of Medicine, Federal University of Ceará, Fortaleza, Brazil. Electronic address: juliana.fernandesp@yahoo.com.br.
  • 7 Post-Graduate Programme in Medical Sciences, Department of Medicine, Federal University of Ceará, Fortaleza, Brazil. Electronic address: emersonoliveira.ferrer@gmail.com.
  • 8 Post-Graduate Programme in Medical Sciences, Department of Medicine, Federal University of Ceará, Fortaleza, Brazil. Electronic address: neilamrl@hotmail.com.
  • 9 Post-Graduate Programme in Pharmacology, Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Brazil. Electronic address: kelly.rose@ufc.br.
  • 10 Post-Graduate Programme in Pharmacology, Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Brazil; Post-Graduate Programme in Medical Sciences, Department of Medicine, Federal University of Ceará, Fortaleza, Brazil; Institute of Biomedicine of Brazilian Semi-arid, Fortaleza, Brazil. Electronic address: gmatos@ufc.br.
Abstract

The pathophysiology of ischemic stroke involves multiple events such as inflammation and oxidative stress which will lead to neuronal death and cognitive deficits. The (-)-α-bisabolol is a monocyclic sesquiterpene alcohol found in various Plants and mainly in Matricaria chamomilla, which exerts antioxidant, anti-inflammatory, and anti-apoptotic activities. The aim of this work was to investigate the neuroprotective effects of (-)-α-bisabolol in mice underwent permanent occlusion of the middle cerebral artery (pMCAO). Animals were treated with (-)-α-bisabolol (50, 100 and 200 mg/kg/day, orally) or vehicle (3% tween 80) one day before and 1 h after pMCAO and the treatment continued once daily for the following five days. The treatment with (-)-α-bisabolol (100 and 200 mg/kg) significantly reduced the infarcted area and neurological deficits caused by pMCAO. (-)-α-bisabolol at the 200 mg/kg dose increased cell viability and decreased neuronal degeneration, as evaluated by cresyl violet and Fluoro-Jade C stainings, respectively. (-)-α-bisabolol also increased the locomotor activity which was reduced by cerebral ischemia and improved pMCAO-induced working, spatial, object recognition, and aversive memories deficits. (-)-α-bisabolol (200 mg/kg) significantly prevented the increase of myeloperoxidase (MPO) activity, TNF-α immunoreactivity in the temporal cortex, and the increase of iNOS both in the temporal cortex and in the striatum. (-)-α-bisabolol treatment also prevented astrogliosis in these areas. These data showed that (-)-α-bisabolol provides neuroprotective action probably due to its anti-inflammatory activity, although other mechanisms cannot be discarded.

Keywords

Brain ischemia; Inflammation; Memory; Neuroprotective effect; α-bisabolol.

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