2. Academic Validation
  3. 2'- O-Methyl-8-methylguanosine as a Z-Form RNA Stabilizer for Structural and Functional Study of Z-RNA

2'- O-Methyl-8-methylguanosine as a Z-Form RNA Stabilizer for Structural and Functional Study of Z-RNA

  • Molecules. 2018 Oct 9;23(10):2572. doi: 10.3390/molecules23102572.
Thananjeyan Balasubramaniyam 1,  Takumi Ishizuka 2,  Chao-Da Xiao 3 4,  Hong-Liang Bao 5,  Yan Xu 6
Affiliations

Affiliations

  • 1 Division of Chemistry, Department of Medical Sciences, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan. balasubramaniyam_thananjeyan@med.miyazaki-u.ac.jp.
  • 2 Division of Chemistry, Department of Medical Sciences, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan. tishizuka@med.miyazaki-u.ac.jp.
  • 3 Division of Chemistry, Department of Medical Sciences, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan. choutatsu_shou@med.miyazaki-u.ac.jp.
  • 4 The key Laboratory of Optimal Utilization of Natural Medicine Resources, School of Pharmaceutical Sciences, Guizhou Medical University, University Town, Guian New District, Guiyang 550025, China. choutatsu_shou@med.miyazaki-u.ac.jp.
  • 5 Division of Chemistry, Department of Medical Sciences, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan. xiaobujiao@163.com.
  • 6 Division of Chemistry, Department of Medical Sciences, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan. xuyan@med.miyazaki-u.ac.jp.
PMID: 30304782 DOI: 10.3390/molecules23102572
Abstract

In contrast to Z-DNA that was stabilized and well-studied for its structure by chemical approaches, the stabilization and structural study of Z-RNA remains a challenge. In this study, we developed a Z-form RNA stabilizer m⁸Gm, and demonstrated that incorporation of m⁸Gm into RNA can markedly stabilize the Z-RNA at low salt conditions. Using the m⁸Gm-contained Z-RNA, we determined the structure of Z-RNA and investigated the interaction of protein and Z-RNA.

Keywords

NMR; Z-RNA structure; circular dichroism; synthesis of oligonucleotide.

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