1. Academic Validation
  2. Monobutyl phthalate (MBP) induces energy metabolism disturbances in the gills of adult zebrafish (Danio rerio)

Monobutyl phthalate (MBP) induces energy metabolism disturbances in the gills of adult zebrafish (Danio rerio)

  • Environ Pollut. 2020 Nov;266(Pt 1):115288. doi: 10.1016/j.envpol.2020.115288.
Yue Tao 1 Yang Yang 1 Yaqi Jiao 1 Song Wu 1 Guangxue Zhu 1 Modupe Sarah Akindolie 1 Tong Zhu 1 Jianhua Qu 1 Lei Wang 1 Ying Zhang 2
Affiliations

Affiliations

  • 1 School of Resources and Environment, Northeast Agricultural University, Harbin, 150030, PR China.
  • 2 School of Resources and Environment, Northeast Agricultural University, Harbin, 150030, PR China. Electronic address: zhangying_neau@163.com.
Abstract

Monobutyl phthalate (MBP) is a primary metabolite of an environmental endocrine disruptor dibutyl phthalate (DBP), which poses a potential threat to living organisms. In this research, the acute toxicity of MBP on energy metabolism in zebrafish gills was studied. Transmission electron microscopy (TEM) results show that 10 mg L-1 MBP can induce mitochondrial structural damage of chloride cells after 96 h of continuous exposure. The activity of ion ATPase and the expression level of oxidative phosphorylation-related genes suggest that MBP interferes with ATP synthesis and ion transport. Further leading to a decrease in mitochondrial membrane potential (MMP) and cell viability, thereby mediating early-stage cell Apoptosis. Through a comprehensive analysis of principal component analysis (PCA) and integrated biomarker response (IBR) scores, atp5a1, a subunit of mitochondrial ATP Synthase, is mainly inhibited by MBP, followed by genes encoding ion ATPase (atp1b2 and atp2b1). Importantly, MBP inhibits aerobic metabolism by inhibiting the key Enzyme malate dehydrogenase (MDH) in the TCA cycle, forcing zebrafish to maintain ATP supply by enhancing anaerobic metabolism.

Keywords

Energy metabolism; Gills damage; Monobutyl phthalate; Zebrafish.

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