1. Academic Validation
  2. A p-toluenesulfonamide-modified benzo[h]chromene hydrazone: Fluorescent turn-on detection of hypochlorite and its application to imaging the endoplasmic reticula of living cells and zebrafishes

A p-toluenesulfonamide-modified benzo[h]chromene hydrazone: Fluorescent turn-on detection of hypochlorite and its application to imaging the endoplasmic reticula of living cells and zebrafishes

  • Spectrochim Acta A Mol Biomol Spectrosc. 2023 Aug 5:296:122669. doi: 10.1016/j.saa.2023.122669.
Yu-Man Tian 1 Gao-Bing Liu 2 Wei-Na Wu 3 Xiao-Lei Zhao 1 Xue-Feng Han 4 Yun-Chang Fan 1 Yuan Wang 5 Zhi-Hong Xu 6
Affiliations

Affiliations

  • 1 College of Chemistry and Chemical Engineering, Henan Key Laboratory of Coal Green Conversion, Henan Polytechnic University, Jiaozuo 454000, PR China.
  • 2 Key Laboratory of Chemo/Biosensing and Detection, College of Chemical and Materials Engineering, Xuchang University, Xuchang 461000, PR China.
  • 3 College of Chemistry and Chemical Engineering, Henan Key Laboratory of Coal Green Conversion, Henan Polytechnic University, Jiaozuo 454000, PR China. Electronic address: wuwn08@hpu.edu.cn.
  • 4 College of Safety Science and Engineering, Henan Polytechnic University, Jiaozuo 454000, PR China.
  • 5 College of Chemistry and Chemical Engineering, Henan Key Laboratory of Coal Green Conversion, Henan Polytechnic University, Jiaozuo 454000, PR China. Electronic address: wangyuan08@hpu.edu.cn.
  • 6 Key Laboratory of Chemo/Biosensing and Detection, College of Chemical and Materials Engineering, Xuchang University, Xuchang 461000, PR China; College of Chemistry, Zhengzhou University, Zhengzhou 450052, PR China. Electronic address: xuzhihong1980@xcu.edu.cn.
Abstract

Hypochlorite (ClO-) is a ROS that plays a crucial role in the immune system in the body. As the largest organelle in the cell, the endoplasmic reticulum (ER) manages various life activities. Thus, a simple hydrazone-based probe was designed, which provided a fast turn-on fluorescent response toward ClO-. With a terminal p-toluenesulfonamide group as the endoplasmic reticulum (ER)-specific site, probe 1 was mainly accumulated at ER of living cells, and could be used for imaging endogenous and exogenous HClO in cells and zebrafishes.

Keywords

Aggregation-induced emission; Biological imaging; Endoplasmic reticulum-targeting; Hydrazone; Hypochlorite.

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