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  2. Butyrate promotes C2C12 myoblast proliferation by activating ERK/MAPK pathway

Butyrate promotes C2C12 myoblast proliferation by activating ERK/MAPK pathway

  • Mol Omics. 2023 May 19. doi: 10.1039/d2mo00256f.
Li Guan 1 Ziyi Cao 1 Ziyue Pan 2 Chao Zhao 3 Mengjuan Xue 4 Fan Yang 5 Jie Chen 1
Affiliations

Affiliations

  • 1 Department of Gastroenterology, Huadong Hospital affiliated to Fudan University, Shanghai, China. laugh_chenjie@fudan.edu.cn.
  • 2 Department of Gastroenterology, Minhang Hospital affiliated to Fudan University, Shanghai, China.
  • 3 Key Laboratory of Medical Molecular Virology, School of Basic Medical sciences, Shanghai Medical College, Fudan university, Shanghai, China.
  • 4 Department of Endocrine, Huadong Hospital affiliated to Fudan University, Shanghai, China.
  • 5 Shanghai Key laboratory of Clinical Geriatric Medicine, Shanghai, China. fdyangfan@fudan.edu.cn.
Abstract

Sarcopenia has garnered considerable interest in recent years as ageing-associated diseases constitute a significant worldwide public health burden. Nutritional supplements have received much attention as potential tools for managing sarcopenia. However, the specific nutrients responsible are still under-investigated. In the current study, we first determined the levels of short chain fatty acids (SCFAs) and intestinal flora in the feces of elderly sarcopenia subjects and elderly healthy individuals by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Then cell viability detection, flow cytometry and transcriptome analysis were adopted to experimentally evaluate the effect and the underlying mechanism of SCFA on C2C12 cells proliferation in vitro. The results suggested that patients with sarcopenia exhibited decreased levels of butyrate. And butyrate may stimulate C2C12 myocyte proliferation via promoting G1/S cell cycle transition. Transcriptomic analyses pointed to upregulation of the Mitogen-activated protein kinase (MAPK) signaling pathway in butyrate-treated cells. In addition, the above proliferative phenotypes could be suppressed by the combination of ERK/MAPK inhibitor. A combined transcriptomic and metabolomic approach was applied in our study to investigate the potential effect of microbiota-derived butyrate yield on muscular proliferation which may indicate a protective effect of nutritional supplements.

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