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  3. Whole-transcriptome analysis after the acquisition of antibiotic resistance of Cronobacter sakazakii: Mechanisms of antibiotic resistance and virulence changes

Whole-transcriptome analysis after the acquisition of antibiotic resistance of Cronobacter sakazakii: Mechanisms of antibiotic resistance and virulence changes

  • Food Res Int. 2023 Dec;174(Pt 2):113664. doi: 10.1016/j.foodres.2023.113664.
Danliangmin Song 1,  Ai Jia 2,  Biqi Liu 3,  Shiyu Liu 4,  Kai Dong 5,  Chaoxin Man 6,  Xinyan Yang 7,  Yujun Jiang 8
Affiliations

Affiliations

  • 1 Key Laboratory of Dairy Science, Ministry of Education, Department of Food Science, Northeast Agricultural University, Harbin, 150030, China. Electronic address: 13161204248@163.com.
  • 2 Key Laboratory of Dairy Science, Ministry of Education, Department of Food Science, Northeast Agricultural University, Harbin, 150030, China. Electronic address: j1825353476@163.com.
  • 3 Key Laboratory of Dairy Science, Ministry of Education, Department of Food Science, Northeast Agricultural University, Harbin, 150030, China. Electronic address: 13206658679@163.com.
  • 4 Key Laboratory of Dairy Science, Ministry of Education, Department of Food Science, Northeast Agricultural University, Harbin, 150030, China. Electronic address: 18103646869@163.com.
  • 5 Key Laboratory of Dairy Science, Ministry of Education, Department of Food Science, Northeast Agricultural University, Harbin, 150030, China. Electronic address: dk283070004@126.com.
  • 6 Key Laboratory of Dairy Science, Ministry of Education, Harbin 150030, China. Electronic address: cxman@neau.edu.cn.
  • 7 Key Laboratory of Dairy Science, Ministry of Education, Harbin 150030, China. Electronic address: 15545116996@163.com.
  • 8 Key Laboratory of Dairy Science, Ministry of Education, Department of Food Science, Northeast Agricultural University, Harbin, 150030, China. Electronic address: yujun_jiang@163.com.
PMID: 37981356 DOI: 10.1016/j.foodres.2023.113664
Abstract

The emergence of antibiotic-resistant bacteria led to the misuse of Antibiotics, resulting in the emergence of more resistant bacteria and continuous improvement in their resistance ability. Cronobacter sakazakii (C. sakazakii) has been considered a pathogen that harms infants. Incidents of C. sakazakii contamination have continued globally, several studies have indicated that C. sakazakii is increasingly resistant to Antibiotics. A few studies have explored the mechanism of Antibiotic resistance in C. sakazakii, and some have examined the Antibiotic resistance and changes in virulence levels. We aimed to investigate the Antibiotic resistance mechanism and virulence differences in C. sakazakii. The level of virulence factors of C. sakazakii was modified after induction by Antibiotics compared with the antibiotic-sensitive strains, and the XS001-Ofl group had the strongest capacity to produce enterotoxin (85.18 pg/mL) and hemolysin (1.47 ng/mL). The biofilm formation capacity after induction significantly improved. The number of Bases and mapped reads in all groups accounted for more than 55 % and 70 %, as detected by transcriptomic analysis. The resistance mechanism of different Antibiotics was more common in efflux pumps, cationic antimicrobial peptides, and biofilm formation pathways. The level of Antibiotic resistance mainly affected the expression of virulence genes associated with flagella assembly and synthesis.

Keywords

Acquisition of antibiotic resistance; Cronobacter sakazakii; Mechanisms of antibiotic resistance; Transcriptome; Virulence differences.

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