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  2. Metallo-β-lactamases immobilized by magnetic zeolitic imidazolate frameworks-8 for degradation of β-lactam antibiotics in an aqueous environment

Metallo-β-lactamases immobilized by magnetic zeolitic imidazolate frameworks-8 for degradation of β-lactam antibiotics in an aqueous environment

  • RSC Adv. 2023 Nov 30;13(49):34884-34890. doi: 10.1039/d3ra05973a.
Quanfang Wang 1 Yuefeng Sun 2 Shidi Zhao 3 Chuqi Bai 1 Zhiwei Cong 4 Yalin Dong 1 Taotao Wang 1
Affiliations

Affiliations

  • 1 Department of Pharmacy, The First Affiliated Hospital of Xi'an Jiaotong University Xi'an 710061 China wangtaotao1989@mail.xjtu.edu.cn dongyalin@mail.xjtu.edu.cn.
  • 2 School of Pharmacy, Xi'an Medical College Xi'an 710021 China.
  • 3 Precision Medicine Center, The First Affiliated Hospital of Xi'an Jiaotong University Xi'an 710061 China.
  • 4 School of Mechanical Engineering, Xi'an Jiaotong University Xi'an 710049 China.
Abstract

Residual Antibiotics in nature are an important cause of antimicrobial drug resistance, and how to deal with residual β-lactam Antibiotics in aqueous environments has become an urgent issue. In this work, magnetic zeolitic imidazolate frameworks-8 (ZIF-8) for immobilizing metallo-β-lactamases (MBLs), or Fe3O4@ZIF-8@MBLs, were successfully synthesized using the one-pot method in aqueous solution. The morphology and chemical structure of Fe3O4@ZIF-8@MBLs were characterized by scanning electron microscopy, energy dispersive spectra, X-ray diffraction, infrared spectra, physical adsorption, and zeta potential. Further, the degradation performance of Fe3O4@ZIF-8@MBLs for β-lactam Antibiotics (penicillin G, cefoperazone, meropenem) in an aqueous environment was investigated by UV-visible absorption spectrophotometry. The results indicated that Fe3O4@ZIF-8@MBLs, compared to control ZIF-8, exhibited superior degradation ability, excellent reusability, and better stability under several harsh conditions. The strategy of combining ZIF-8 and MBLs to form magnetic porous Polymers may be suitable for removing β-lactam Antibiotics from an aqueous environment. This work provided an original insight into future studies on the degradation of β-lactam Antibiotics employing MBLs immobilized by magnetic metal-organic frameworks.

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