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  2. Melatonin alleviates chronic stress-induced hippocampal microglia pyroptosis and subsequent depression-like behaviors by inhibiting Cathepsin B/NLRP3 signaling pathway in rats

Melatonin alleviates chronic stress-induced hippocampal microglia pyroptosis and subsequent depression-like behaviors by inhibiting Cathepsin B/NLRP3 signaling pathway in rats

  • Transl Psychiatry. 2024 Mar 27;14(1):166. doi: 10.1038/s41398-024-02887-y.
Zhicheng Gao # 1 Kangxin Luo # 1 Yulin Hu 1 Yunqian Niu 1 Xinchao Zhu 1 Shoujun Li 2 Haiyang Zhang 3
Affiliations

Affiliations

  • 1 College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, PR China.
  • 2 College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, PR China. shoujunli@scau.edu.cn.
  • 3 College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, PR China. haiyangzhang2021@scau.edu.cn.
  • # Contributed equally.
Abstract

Melatonin improves chronic stress-induced hippocampal damage and depression-like behaviors, but the mechanism needs further study. This study was to explore the mechanism of melatonin inhibiting microglia Pyroptosis. In virtro experiments, melatonin improved corticosterone-induced the ultrastructure and microstructure damage of HAPI cells by inhibiting Pyroptosis, thereby increasing cell survival rate. Protein-protein interaction network and molecular autodocking predicted that Cathespin B might be the target of melatonin inhibition of NLRP3-mediated Pyroptosis. Melatonin inhibited corticosterone-induced Cathespin B expression. Both Cathepsin B Inhibitor CA-074Me and NLRP3 knockout inhibited the HAPI cells Pyroptosis. Similarly, melatonin inhibited Cathepsin B agonist Pazopanib-induced activation of Cathepsin B/NLRP3 signaling pathway and HAPI cells Pyroptosis. In vivo studies, melatonin inhibited chronic restraint stress (CRS)-induced activation of Cathepsin B/NLRP3 signaling pathway and alleviated hippocampal microglia Pyroptosis in rats. Inhibition of microglia Pyroptosis improved CRS-induced depression-like behaviors of rats. In addition, inhibition of Cathepsin B and NLRP3 alleviated hippocampal Pyroptosis. Melatonin inhibited Pazopanib-induced activation of Cathepsin B/NLRP3 signaling pathway and hippocampal Pyroptosis. These results demonstrated that melatonin could alleviate CRS-induced hippocampal microglia Pyroptosis by inhibiting Cathepsin B/NLRP3 signaling pathway, thereby improving depression-like behaviors in rats. This study reveals the molecular mechanism of melatonin in the prevention and treatment of chronic stress-related encephalopathy.

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