2. Academic Validation
  3. Peptide REF1 is a local wound signal promoting plant regeneration

Peptide REF1 is a local wound signal promoting plant regeneration

  • Cell. 2024 Jun 6;187(12):3024-3038.e14. doi: 10.1016/j.cell.2024.04.040.
Wentao Yang 1,  Huawei Zhai 2,  Fangming Wu 3,  Lei Deng 4,  Yu Chao 3,  Xianwen Meng 2,  Qian Chen 2,  Chenhuan Liu 5,  Xiaomin Bie 6,  Chuanlong Sun 2,  Yang Yu 7,  Xiaofei Zhang 5,  Xiaoyue Zhang 3,  Zeqian Chang 3,  Min Xue 8,  Yajie Zhao 6,  Xiangbing Meng 9,  Boshu Li 10,  Xiansheng Zhang 6,  Dajian Zhang 8,  Xiangyu Zhao 6,  Caixia Gao 10,  Jiayang Li 9,  Chuanyou Li 11
Affiliations

Affiliations

  • 1 Key Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; Taishan Academy of Tomato Innovation, Shandong Agricultural University, Tai'an 271018, Shandong, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; College of Life Sciences, Shandong Agricultural University, Tai'an 271018, Shandong, China.
  • 2 Taishan Academy of Tomato Innovation, Shandong Agricultural University, Tai'an 271018, Shandong, China; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, Shandong, China.
  • 3 Key Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; Taishan Academy of Tomato Innovation, Shandong Agricultural University, Tai'an 271018, Shandong, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
  • 4 Taishan Academy of Tomato Innovation, Shandong Agricultural University, Tai'an 271018, Shandong, China; College of Life Sciences, Shandong Agricultural University, Tai'an 271018, Shandong, China. Electronic address: ldeng@sdau.edu.cn.
  • 5 Key Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
  • 6 College of Life Sciences, Shandong Agricultural University, Tai'an 271018, Shandong, China.
  • 7 Taishan Academy of Tomato Innovation, Shandong Agricultural University, Tai'an 271018, Shandong, China; College of Life Sciences, Shandong Agricultural University, Tai'an 271018, Shandong, China.
  • 8 College of Agronomy, Shandong Agricultural University, Tai'an 271018, Shandong, China.
  • 9 Key Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
  • 10 College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; New Cornerstone Science Laboratory, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
  • 11 Key Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; Taishan Academy of Tomato Innovation, Shandong Agricultural University, Tai'an 271018, Shandong, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; College of Life Sciences, Shandong Agricultural University, Tai'an 271018, Shandong, China; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, Shandong, China. Electronic address: cyli@genetics.ac.cn.
PMID: 38781969 DOI: 10.1016/j.cell.2024.04.040
Abstract

Plants frequently encounter wounding and have evolved an extraordinary regenerative capacity to heal the wounds. However, the wound signal that triggers regenerative responses has not been identified. Here, through characterization of a tomato mutant defective in both wound-induced defense and regeneration, we demonstrate that in tomato, a plant elicitor peptide (Pep), REGENERATION FACTOR1 (REF1), acts as a systemin-independent local wound signal that primarily regulates local defense responses and regenerative responses in response to wounding. We further identified PEPR1/2 ORTHOLOG RECEPTOR-LIKE KINASE1 (PORK1) as the receptor perceiving REF1 signal for plant regeneration. REF1-PORK1-mediated signaling promotes regeneration via activating WOUND-INDUCED DEDIFFERENTIATION 1 (WIND1), a master regulator of wound-induced cellular reprogramming in Plants. Thus, REF1-PORK1 signaling represents a conserved phytocytokine pathway to initiate, amplify, and stabilize a signaling cascade that orchestrates wound-triggered organ regeneration. Application of REF1 provides a simple method to boost the regeneration and transformation efficiency of recalcitrant crops.

Keywords

REF1; REGENERATION FACTOR1; local defense responses; phytocytokine; plant elicitor peptide; regeneration; systemic defense responses; tomato; wound responses; wound signal.

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