2. Academic Validation
  3. Dauriporphine inhibited lung cancer cell viability, motility, and energy metabolism through the miR-424-5p/MAPK14 axis

Dauriporphine inhibited lung cancer cell viability, motility, and energy metabolism through the miR-424-5p/MAPK14 axis

  • Hereditas. 2025 Jun 11;162(1):101. doi: 10.1186/s41065-025-00473-w.
Yan-Jia Du # 1 2,  Jin-Peng Lv # 3,  Yao Fu 1,  Meng Lan 1 2,  Jing-Feng Li 4,  Hui Zhang 5,  Nan Wu 6 7
Affiliations

Affiliations

  • 1 Department of Chinese Medicine and Health Care, Changchun Humanities and Sciences College, No. 1488, Boshuo Road, Jingyue National Hi-Tech Industrial Development Zone, Changchun, 130117, Jilin Province, China.
  • 2 Jilin Northeast Asia Biotechnology Development Co., Ltd, Room 16-3-105, Luwafangfu'ao I, Jingyue Development Zone, Changchun City, Jilin Province, 130033, China.
  • 3 Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, 130022, China.
  • 4 College of Pharmacy, Changchun University of Chinese Medicine, Changchun, 130117, China.
  • 5 Department of Chinese Medicine and Health Care, Changchun Humanities and Sciences College, No. 1488, Boshuo Road, Jingyue National Hi-Tech Industrial Development Zone, Changchun, 130117, Jilin Province, China. zhanxyz08@163.com.
  • 6 Department of Chinese Medicine and Health Care, Changchun Humanities and Sciences College, No. 1488, Boshuo Road, Jingyue National Hi-Tech Industrial Development Zone, Changchun, 130117, Jilin Province, China. dyj71366@163.com.
  • 7 Jilin Northeast Asia Biotechnology Development Co., Ltd, Room 16-3-105, Luwafangfu'ao I, Jingyue Development Zone, Changchun City, Jilin Province, 130033, China. dyj71366@163.com.
  • # Contributed equally.
PMID: 40500789 DOI: 10.1186/s41065-025-00473-w
Abstract

Background: Dauriporphine is a major ingredient of Manispernum daericum DC., which has been demonstrated to show wide anti-tumor activities. miR-424-5p, as a regulator of lung Cancer, was hypothesized to serve as the therapeutic target for dauriporphine This study evaluated the potential of dauriporphine in treating lung adenocarcinoma and revealed the underlying molecular mechanism.

Results: The anti-tumor effect of dauriporphine on lung adenocarcinoma was assessed in A549 cells, and it was found that dauriporphine significantly inhibited the viability of A549 cells in a concentration-dependent manner with the half maximal inhibitory concentration (IC50) value of 10.57 µM. Dauriporphine induced decreasing cell growth, motility, and energy metabolism, indicating the anti-tumor effect of dauriporphine on A549 cells. Dauriporphine inducing elevated miR-424-5p levels, while silencing miR-424-5p significantly recovered cell viability, migration, and energy metabolism of A549 cells. Mitogen-activated protein Kinase 14 (MAPK14) was negatively regulated by miR-424-5p, and the knockdown of MAPK14 could reverse the protective effect of miR-424-5p on dauriporphine-treated A549 cells.

Conclusion: Dauriporphine inhibited cell growth, metastasis, and glycolysis-related energy metabolism of lung adenocarcinoma cells via modulating miR-424-5p/MAPK14 axis. Dauriporphine can be considered in drug development for lung adenocarcinoma.

Clinical trial number: Not applicable.

Keywords

Anti-lung cancer treatment; Cell motility; Cell viability; Dauriporphine; Energy metabolism.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z