2. Academic Validation
  3. 3D collagen nanofiber scaffold with adipose derived stem cells for functional adipose tissue regeneration

3D collagen nanofiber scaffold with adipose derived stem cells for functional adipose tissue regeneration

  • Sci Rep. 2025 Jul 1;15(1):21802. doi: 10.1038/s41598-025-04763-1.
Liping Zhang # 1 2,  Yueguang Xue # 3 4,  Delu Mu # 1 2,  Siyi Chen 1,  Guangmin Wu 1 2,  Zitong Yang 1,  Yizhu Chen 1,  Hu Xu 1,  Li Zhu 5,  Jiandong Wang 6 7
Affiliations

Affiliations

  • 1 Department of General Surgery, The First Medical Center of PLA General Hospital, Beijing, 100853, China.
  • 2 Inner Mongolia Medical University, Hohhot, 010110, Inner Mongolia, China.
  • 3 School of Biomedical Sciences and Engineering, South China University of Technology, Guangzhou, 511442, Guangdong, P.R. China.
  • 4 Center for Excellence in Nanoscience, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS, National Center for Nanoscience and Technology of China, Beijing, 100190, P.R. China.
  • 5 Department of General Surgery, The First Medical Center of PLA General Hospital, Beijing, 100853, China. zhuxiaoli0430@163.com.
  • 6 Department of General Surgery, The First Medical Center of PLA General Hospital, Beijing, 100853, China. vicky1968@163.com.
  • 7 Inner Mongolia Medical University, Hohhot, 010110, Inner Mongolia, China. vicky1968@163.com.
  • # Contributed equally.
PMID: 40593981 DOI: 10.1038/s41598-025-04763-1
Abstract

Adipose tissue engineering offers a promising approach for breast reconstruction, yet achieving efficient adipose regeneration remains challenging due to poor cell survival and tissue integration. Hence, we developed a three-dimensional (3D) electrospun Collagen nanofiber scaffold integrated with adipose-derived mesenchymal stem cells (ADSCs), designed to enhance adipose tissue regeneration by providing a biomimetic extracellular matrix environment. The incorporation of Collagen nanofibers enhances cell adhesion and extracellular matrix remodeling, further promoting adipogenic differentiation. Compared to conventional two-dimensional (2D) culture, ADSCs seeded on the scaffold exhibit significantly improved viability and lipid accumulation. In vivo implantation in a rat model demonstrated that the COL-ADSCs composite scaffold increased subcutaneous fat thickness from 2.69 ± 0.10 mm to 3.37 ± 0.11 mm over four weeks, while also promoting Collagen remodeling and angiogenesis, as confirmed by CD31-positive staining. Despite these promising outcomes, this study is limited to a small animal model, and further validation in large animal models and clinical settings is necessary. These findings indicate that the COL-ADSCs composite scaffold provides a biomimetic microenvironment that supports ADSC adhesion, differentiation, and tissue remodeling, highlighting its potential as a clinically applicable biomaterial for breast reconstruction.

Keywords

Adipose tissue engineering; Adipose-derived mesenchymal stem cells; Breast reconstruction; Composite biopatch; Electrostatic spinning scaffolds.

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