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  3. Multi-omics and network pharmacology reveal Huayu-Tongbi decoction reduced arthritis-related bone erosion

Multi-omics and network pharmacology reveal Huayu-Tongbi decoction reduced arthritis-related bone erosion

  • Chin Med. 2025 Jul 2;20(1):100. doi: 10.1186/s13020-025-01159-1.
Bozhen Chen # 1 2,  Lu Yang # 1 2,  Houchun Wang 1,  Peng Yu 1 2,  Mengyang Ma 1 3,  Meiqi Chen 1 2,  Yingyan Zhou 1 2 4 5,  Jiaqi Wu 1 2 4 5,  Huasheng Liang 1,  Maojie Wang 1 2 6 5,  Runyue Huang 1 2 6 5,  Yiting He 1 2 7 5,  Qingchun Huang 1 2 6 5,  Xiaohong He 8 9 10 11
Affiliations

Affiliations

  • 1 Second Clinical Medical College of Guangzhou University of Chinese Medicine, No.111, Dade Road, Yuexiu District, Guangzhou City, 510120, Guangdong, People's Republic of China.
  • 2 Guangdong-Hong Kong-Macau Joint Lab on Chinese Medicine and Immune Disease Research, Guangzhou University of Chinese Medicine, Guangzhou, China.
  • 3 Luoyang Orthopedic Hospital of Henan Province & Orthopedic Hospital of Henan Province, Luoyang, Henan, China.
  • 4 Guangdong Provincial Key Laboratory of Chinese Medicine for Prevention and Treatment of Refractory Chronic Diseases, Guangzhou, China.
  • 5 The Second Affiliated Hospital, Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine), Guangzhou, China.
  • 6 Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome, Guangzhou, China.
  • 7 State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine), Guangzhou, China.
  • 8 Second Clinical Medical College of Guangzhou University of Chinese Medicine, No.111, Dade Road, Yuexiu District, Guangzhou City, 510120, Guangdong, People's Republic of China. wdtsxh521@126.com.
  • 9 Guangdong-Hong Kong-Macau Joint Lab on Chinese Medicine and Immune Disease Research, Guangzhou University of Chinese Medicine, Guangzhou, China. wdtsxh521@126.com.
  • 10 Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome, Guangzhou, China. wdtsxh521@126.com.
  • 11 The Second Affiliated Hospital, Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine), Guangzhou, China. wdtsxh521@126.com.
  • # Contributed equally.
PMID: 40598199 DOI: 10.1186/s13020-025-01159-1
Abstract

Background: Rheumatoid arthritis (RA), an autoimmune disorder marked by joint inflammation and bone destruction, lacks effective therapies targeting bone erosion. Huayu-Tongbi decoction (HT), a traditional Chinese medicine (TCM) herbal decoction, has been used as a complementary treatment for RA, yet the mechanisms of its active components and multitarget therapeutic effects remain unclear.

Materials and methods: An adjuvant-induced arthritis (AIA) model was established in rats, and enzyme-linked immunosorbent assay, histopathological staining, and micro-Computed Tomography to assess the effects of HT on joint inflammation and bone erosion. Furthermore, serum pharmacochemistry combined with network pharmacology identified the HT's active ingredients and targets. In vitro multi-omics study revealed the decoction's effect and underlying mechanisms in osteoclastic differentiation.

Results: HT significantly reduced joint inflammation and bone erosion in AIA rats. Serum pharmacochemistry identified 44 absorbed components in HT, and network pharmacology analysis predicted 89 key targets of HT related to RA. In vitro experiments demonstrated that HT inhibited RANKL-induced osteoclastic differentiation through multiple pathways, such as PPAR pathway, AA metabolism, and NF-κB pathway.

Conclusion: This study confirmed the beneficial effects of HT in experimental arthritis and explored the specific mechanisms involved. HT inhibited osteoclastic differentiation through multiple targets and pathways to reduced bone destructions, providing a potential therapeutic strategy for preventing RA-related bone erosion.

Keywords

Bone erosion; Huayu-Tongbi decoction; Multiomics; Network pharmacology; Rheumatoid arthritis; Traditional Chinese Medicine.

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