2. Academic Validation
  3. Macrophage-mimetic liposomes co-delivering ceria and Ac2-26 peptide for penumbra protection in ischemic stroke

Macrophage-mimetic liposomes co-delivering ceria and Ac2-26 peptide for penumbra protection in ischemic stroke

  • J Nanobiotechnology. 2025 Jul 5;23(1):488. doi: 10.1186/s12951-025-03575-9.
Lingyun Bai # 1,  Na Li # 2,  Huiqin Li # 3,  Yuxuan Dai 3,  Yi Bai 1,  Feiyu Ma 1,  Dujuan Sha 4,  Shengnan Xia 3,  Yun Xu 5 6,  Xiang Cao 7 8 9
Affiliations

Affiliations

  • 1 Department of Neurology, Nanjing Drum Tower Hospital, Drum Tower Hospital Life and Health Research Institute, Nanjing Normal University, Nanjing, 210008, China.
  • 2 Department of Blood Transfusion Medicine, Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, 210002, China.
  • 3 Department of Neurology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, 210008, China.
  • 4 Department of General Practice, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, 210008, China.
  • 5 Department of Neurology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, 210008, China. xuyun20042001@aliyun.com.
  • 6 Nanjing Neurology Clinical Medical Center, Nanjing, 210008, China. xuyun20042001@aliyun.com.
  • 7 Department of Neurology, Nanjing Drum Tower Hospital, Drum Tower Hospital Life and Health Research Institute, Nanjing Normal University, Nanjing, 210008, China. caoxiang@njglyy.com.
  • 8 Department of Neurology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, 210008, China. caoxiang@njglyy.com.
  • 9 Nanjing Neurology Clinical Medical Center, Nanjing, 210008, China. caoxiang@njglyy.com.
  • # Contributed equally.
PMID: 40618146 DOI: 10.1186/s12951-025-03575-9
Abstract

Ischemic stroke is one of the most severe central nervous system disease with high disability and mortality rates worldwide. Effective treatment requires strategies that address the multifaceted pathophysiology of ischemic penumbra, including oxidative stress-induced neuronal Apoptosis and blood-brain barrier (BBB) disruption. Here, inspired by the inflammation-homing property of macrophages, we developed macrophage-mimetic liposomes (MM-LPs) by integrating macrophage membranes with liposomes for the co-delivery of cerium oxide nanoparticles (CeO2) and the Ac2-26 peptide (CeO2-Ac2-26@MM-LPs). CeO2 effectively scavenged Reactive Oxygen Species (ROS), improving mitochondrial function and inhibiting neuronal Apoptosis, while Ac2-26 upregulated tight junction proteins to enhance BBB integrity. In a transient middle cerebral artery occlusion (tMCAO) mouse model, CeO2-Ac2-26@MM-LPs exhibited significant accumulation in ischemic regions, where they exerted dual protective effects on the ischemic penumbra by mitigating neuronal damage and preserving BBB integrity. This resulted in improved neurological function, reduced infarct volume, and attenuated BBB disruption. Mechanistically, CeO2-Ac2-26@MM-LPs activated the NRF2 signaling pathway, upregulating antioxidant Enzymes (HO-1 and NQO1), while simultaneously suppressing NF-κB activation, thereby reducing neuronal death and BBB damage in the penumbra. This multi-target combination strategy provides a promising platform for ischemic stroke treatment, with potential applicability to Other neurological disorders characterized by oxidative stress and BBB disruption.

Keywords

Blood-brain barrier; Ceria; Ischemic stroke; Macrophage membrane; Neurons; Reactive oxygen species.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z