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  2. Natural dietary polyphenolic compounds cause endothelium-dependent vasorelaxation in rat thoracic aorta

Natural dietary polyphenolic compounds cause endothelium-dependent vasorelaxation in rat thoracic aorta

  • J Nutr. 1998 Dec;128(12):2324-33. doi: 10.1093/jn/128.12.2324.
E Andriambeloson 1 C Magnier G Haan-Archipoff A Lobstein R Anton A Beretz J C Stoclet R Andriantsitohaina
Affiliations

Affiliation

  • 1 Laboratoire de Pharmacologie et Physiopathologie Cellulaires, Universite Louis Pasteur de Strasbourg, CNRS ERS 653 Faculte de Pharmacie, BP 24, 67401 Illkirch-Cedex, France.
Abstract

This study investigated the possible active principles which support the endothelial nitric oxide-dependent relaxation produced by red wine and other plant polyphenolic compounds in thoracic aorta from male Wistar rats (12-14 wk old). Relaxation experiments were recorded isometrically on vessels precontracted with norepinephrine. Ten different chromatographic fractions (3-18 mg) isolated from red wine polyphenolic compounds (RWPC) and some available defined Polyphenols (10-15 mg) were tested. Fractions enriched into either anthocyanins or oligomeric condensed tannins exhibited endothelium-dependent vasorelaxant activity (maximal relaxation in the range of 59-77%) comparable to the original RWPC. However, polymeric condensed tannins elicited a weaker vasorelaxant activity than the original RWPC (maximal relaxation ranged between 20-47%, P < 0.01). Moreover, the representative of either phenolic acid derivatives (benzoic acid, vanillic acid, gallic acid), hydroxycinnamic acid (p-coumaric acid, caffeic acid) or the flavanol [(+)-epicatechin] classes failed to induce this type of response. Among the anthocyanins, delphinidin (maximal relaxation being 89%), but not malvidin or cyanidin, showed endothelium-dependent vasorelaxation. These results show that anthocyanins and oligomeric-condensed tannins exhibited a pharmacological profile comparable to the original RWPC. These compounds may be involved in the reduction of cardiovascular mortality related to the presence of wine, fruits and vegetables in the diet.

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