2. 重组蛋白
  3. Cytokines and Growth Factors
  4. CSF & Receptors
  5. GM-CSF
  6. GM-CSF 蛋白, Human (HEK293)

GM-CSF 蛋白, Human (HEK293)

目录号: HY-P73080
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3.  试用装只面向终端客户。

规格 价格 是否有货 数量
20 μg ￥4550 In-stock
50 μg   询价  
100 μg   询价  

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Top Publications Citing Use of Products

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

生物活性

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells and the ED50 is typically 0.1-0.6 ng/mL.
种属

Human
表达系统

HEK293
标签

Tag Free
蛋白编号

P04141 (M1-E144)
基因 ID

1437  [NCBI]
同用名
Granulocyte-macrophage colony-stimulating factor; GM-CSF; CSF; CSF-2
氨基酸序列

MWLQSLLLLGTVACSISAPARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE

分子量

Approximately 14.5 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE
性状

Lyophilized powder.
组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4
内毒素含量

<1 EU/μg, determined by LAL method.
复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
运输条件

Room temperature in continental US; may vary elsewhere.
文件资料
产品使用指南 (1213 KB)

GM-CSF Protein, Human (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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