SMARCA1 抗体 (YA3932)

SMARCA1 Antibody (YA3932) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SMARCA1.

SMARCA1 是一种具有内在 ATP 依赖性染色质重塑活性的 ATPase (PubMed:14609955，PubMed:15310751，PubMed:15640247，PubMed:28801535)。其 ATPase 活性依赖于底物，当核小体为底物时活性增强，但也能以 DNA 为底物催化 (PubMed:14609955，PubMed:15310751，PubMed:15640247)。作为 ISWI 染色质重塑复合物的催化亚基，这些复合物在染色质上形成有序的核小体阵列，并在 DNA 复制、转录和修复等 DNA 模板化过程中促进 DNA 的可及性 (PubMed:14609955，PubMed:15310751，PubMed:15640247，PubMed:28801535)。在 ISWI 染色质重塑复合物中，SMARCA1 将边缘和中心定位的组蛋白八聚体从 DNA 模板上的原始位置滑离 (PubMed:28801535)。其催化活性和组蛋白八聚体滑动倾向受 ISWI 染色质重塑复合物组分的调控 (PubMed:28801535)。含有 BAZ1A、BAZ1B、BAZ2A 和 BAZ2B 的 ISWI 染色质重塑复合物调控核小体在染色质上的间距，并能将单核小体滑动至 DNA 模板的中心 (PubMed:28801535)。而含有 CECR2 和 RSF1 的 ISWI 染色质重塑复合物则不具备此能力 (PubMed:28801535)。在 NURF-1 和 CERF-1 ISWI 染色质重塑复合物中，核小体是其 ATPase 活性的首选底物 (PubMed:14609955，PubMed:15640247)。在 NURF-1 ISWI 染色质重塑复合物中，SMARCA1 结合 En1 和 En2 的启动子，正向调控其表达并促进大脑发育 (PubMed:14609955)。可能促进神经突生长 (PubMed:14609955)。可能参与黄体细胞的发育 (PubMed:16740656)。在 DNA 复制过程中促进核小体组装，通过防止复制应激和新 DNA 缺口确保复制叉的进展和基因组稳定性 (PubMed:39413208)。当底物为 DNA 或核小体时，SMARCA1 催化失活且不具备染色质重塑活性 (PubMed:15310751，PubMed:28801535)。通过生成无活性复合物，作为染色质重塑的负调控因子 (PubMed:15310751)。

P28370

Predicted band size: 123 kDa; Observed band size: 123 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse

WB: 1:500-1:2000; IHC: 1:200-1:1000; FC: 1:200-1:400; ELISA: 1:10000

• 
  Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using SMARCA1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P84235, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using SMARCA1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P84235, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, FC, ELISA

液体

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human SMARCA1 (AA: 933-1070) expressed in E. Coli.