1. 染料试剂

Dye Reagents

A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.

Dye Reagents (332):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-D0940
    H2DCFDA 4091-99-0 99.82%
    H2DCFDA (DCFH-DA) 是一种可渗透细胞的,用于检测细胞内活性氧 (ROS) 的探针。
    H2DCFDA
  • HY-D0938
    CFSE 150347-59-4 98.26%
    CFSE (5(6)-Carboxyfluorescein diacetate succinimidyl ester) 是一种细胞内荧光染料,可追踪细胞增殖。共价结合的 CFSE 在子细胞之间平均分配,从而可以区分连续的细胞分裂。
    CFSE
  • HY-15534
    JC-1 3520-43-2 99.46%
    JC-1 (CBIC2) 是荧光亲脂性羰花青染料,用于测量线粒体膜电位。线粒体膜电位较高时, JC-1 在基质中汇聚形成聚合物 (J-aggregates),可以产生红色荧光 (Ex/Em=585/590 nm);线粒体膜电位较低时,JC-1 不能聚集在线粒体基质中,以单体形式存在产生绿色荧光 (Ex/Em=510/527 nm)。
    JC-1
  • HY-15924
    Thiazolyl Blue 298-93-1 99.84%
    Thiazolyl Blue (MTT) 是一种广泛用于测量细胞增殖的比色剂。Thiazolyl Blue (MTT) 在活细胞中从黄色还原为紫色甲。
    Thiazolyl Blue
  • HY-12591B
    D-Luciferin potassium salt 115144-35-9 99.93%
    D-Luciferin potassium salt 是萤光素酶的底物,可催化生物发光昆虫产生典型的黄绿光。
    D-Luciferin potassium salt
  • HY-D0815
    Propidium Iodide 25535-16-4 99.68%
    Propidium Iodide 是可用于细胞染色的红色荧光染料。
    Propidium Iodide
  • HY-D0814
    DAPI dihydrochloride 28718-90-3 >98.0%
    DAPI dihydrochloride 是结合富含 A-T 碱基对 DNA 序列的荧光染色。
    DAPI dihydrochloride
  • HY-D0718
    Nile Red 7385-67-3 98.02%
    Nile Red (Nile Blue A oxazone) 是一种针对细胞内脂质小滴和中性脂质的选择性疏水性荧光染料。Nile Red 在所有有机溶剂中均具有强烈的荧光性,其荧光颜色范围从金黄色到深红色。
    Nile Red
  • HY-D0041
    Calcein-AM 148504-34-1 >98.0%
    Calcein-AM是用于测定细胞活力的可以渗透细胞的荧光染料。
    Calcein-AM
  • HY-12591A
    D-Luciferin 2591-17-5 98.81%
    D-Luciferin 是萤光素酶的底物,可催化生物发光昆虫产生典型的黄绿光。
    D-Luciferin
  • HY-111382
    Diphenylterazine 344940-63-2 98.06%
    Diphenylterazine (DTZ) 是一种生物发光剂。Diphenylterazine 本身仅产生很少的背景,导致极好的信噪比。
    Diphenylterazine
  • HY-66019
    FITC 3326-32-7 >98.0%
    FITC (Fluorescein 5-isothiocyanate) 是一种用于胺标记的荧光探针。FITC 是对 pH 和 Cu2+ 敏感的荧光染料。
    FITC
  • HY-15559A
    Hoechst 33342 trihydrochloride 875756-97-1 99.87%
    Hoechst 33342 trihydrochloride是可以膜透细胞膜,发蓝色荧光的 DNA 染料。
    Hoechst 33342 trihydrochloride
  • HY-D0985A
    TMRE 115532-52-0 98.24%
    TMRE 是一种线粒体特异性染料 (λex=550 nm,λem=575 nm)。
    TMRE
  • HY-D0711
    Cardiogreen 3599-32-4 98.23%
    Cardiogreen是一种低毒性荧光剂,广泛用于医学诊断,如确定心输出量,肝功能和肝血流量,以及用于眼科血管造影。
    Cardiogreen
  • HY-D0083
    DiI 41085-99-8 99.92%
    DiI (DiIC18(3)) 是一种亲脂性膜染料。DiI 在进入细胞膜之前荧光非常弱,仅当进入到细胞膜后才可以被激发出很强的荧光。DiI 被激发后可以发出橙红色的荧光,最大激发波长为 549 nm,最大发射波长为 565 nm。通常用作神经元和其它细胞的长期示踪剂。
    DiI
  • HY-128868
    FITC-Dextran (MW 10000) 60842-46-8
    FITC-Dextran (MW 10000) 是由 FITC 偶联葡聚糖形成的一种标记物 (10 kDa)。FITC-Dextran 是由不同长度的支链葡萄糖分子组成的被标记的多糖,可根据所使用的葡聚糖的大小来测定血脑屏障的溶质、离子和蛋白质渗透性。
    FITC-Dextran (MW 10000)
  • HY-D0831
    WST-8 193149-74-5 >98.0%
    WST-8 是一种水溶性的四唑染料,相对于常规MTS检测,WTS-8测定法的灵敏度增强。
    WST-8
  • HY-D0218
    Thioflavin T 2390-54-7 98.72%
    Thioflavin T 是一种阳离子 Benzothiazole 染料,在组织切片中与淀粉样蛋白结合后荧光强度增强。
    Thioflavin T
  • HY-D0079
    Dihydroethidium 104821-25-2 >98.0%
    Dihydroethidium(Hydroethidine; PD-MY 003)是过氧化物指示剂,在细胞质中具有蓝色荧光,直到被氧化。
    Dihydroethidium