1. MCE Kits
  2. Rhodamine Phalloidin

Rhodamine Phalloidin 

Cat. No.: HY-K0903
Manual SDS

罗丹明标记鬼笔环肽是一种高亲和的 F-肌动蛋白探针,与橙色染料四甲基罗丹明 (TRITC) 偶联。

Rhodamine Phalloidin

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

Size Price Stock
300T ¥4800 Ask For Quote & Lead Time

* Please select Quantity before adding items.

  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

Phalloidin is a mushroom-derived toxin which can be used to specifically label F-actins (actin filaments) of the cytoskeleton. It binds to all variants of actin filaments in many different species of animals and plants. Typically, Phalloidin is used conjugated to a fluorescent dye. Phalloidin binds F-actins with high selectivity while Rhodamine provides stable and bright orange fluorescence.

Rhodamine Phalloidin is 1000× stock solution in DMSO which is convenient for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments.

Storage

The solutions should be stable for at least 6 months if store at -20°C.

Protecting from light, and avoid freeze/thaw cycles.

Protocol

1.   Prepare 1× Rhodamine Phalloidin working solution:

Add 1 μL of 1000× Rhodamine Phalloidin DMSO solution to 1 mL of PBS containing with 1% BSA.

Note: 1. Different cell types might be stained differently. The concentration of Rhodamine Phalloidin working solution should be prepared accordingly.

          2. 1% bovine serum albumin (BSA) is used to reduce nonspecific background staining.

2.   Stain the cells:

2.1   Wash cells 2–3 times with PBS. Fix the cells in 3.7% methanol-free formaldehyde solution in PBS for 10-30 minutes at room temperature.

Note: Avoid any methanol containing fixatives since methanol can disrupt actin during the fixation process. The preferred fixative is methanol-free formaldehyde.

2.2   Wash the fixed cells 2–3 times in PBS.

2.3   Optional: Add 0.1% Triton X-100 in PBS into fixed cells for 3–5 minutes to increase permeability. Wash the cells 2–3 times in PBS.

2.4   Add 100 μL/well (96-well plate) of Rhodamine Phalloidin working solution into the fixed cells, and stain the cells at room temperature for 20 to 90 minutes.

2.5   Rinse cells gently with PBS 2–3 times to remove excess Rhodamine Phalloidin.

2.6   Run fluorescence microscope or other equipments at desired Ex/Em wavelengths (Rhodamine Phalloidin, Ex/Em=552/578 nm).

Components
Cat. No. Product Name Ex (nm) Em (nm) Size Package
HY-K0903 Rhodamine Phalloidin 552.0 578.0 300 T 30 μL
Documentation

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产品名称:
Rhodamine Phalloidin
目录号:
HY-K0903
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