1. Academic Validation
  2. Biodecolorization of a food azo dye by the deep sea Dermacoccus abyssi MT1.1(T) strain from the Mariana Trench

Biodecolorization of a food azo dye by the deep sea Dermacoccus abyssi MT1.1(T) strain from the Mariana Trench

  • J Environ Manage. 2014 Jan;132:155-64. doi: 10.1016/j.jenvman.2013.11.002.
Weeranuch Lang 1 Sarote Sirisansaneeyakul 2 Lígia O Martins 3 Lukana Ngiwsara 1 Nobuo Sakairi 4 Wasu Pathom-aree 5 Masayuki Okuyama 1 Haruhide Mori 1 Atsuo Kimura 6
Affiliations

Affiliations

  • 1 Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
  • 2 Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand; Centre for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart University, Bangkok 10900, Thailand. Electronic address: sarote.s@ku.ac.th.
  • 3 Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras 2780-157, Portugal.
  • 4 Graduate School of Environmental Science, Hokkaido University, Sapporo 060-0810, Japan.
  • 5 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.
  • 6 Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan. Electronic address: kimura@abs.agr.hokudai.ac.jp.
Abstract

This study reports the characterization of the ability of Dermacoccus spp. isolated from the deepest point of the world's oceans for azo dye decolorization. A detailed investigation of Dermacoccus abyssi MT1.1(T) with respect to the azoreductase activity and enzymatic mechanism as well as the potential role of the Bacterial strain for biocleaning of industrial dye baths is reported. Resting cells with oxygen-insensitive azoreductase resulted in the rapid decolorization of the polysulfonated dye Brilliant Black BN (BBN) which is a common food colorant. The highest specific decolorization rate (vs) was found at 50 °C with a moderately thermal tolerance for over 1 h. Kinetic analysis showed the high rates and strong affinity of the enzymatic system for the dye with a Vmax = 137 mg/g cell/h and a Km = 19 mg/L. The degradation of BBN produces an initial orange intermediate, 8-amino-5-((4-sulfonatophenyl)diazenyl)naphthalene-2-sulfonic acid, identified by mass spectrometry which is later converted to 4-aminobenzene sulfonic acid. Nearly 80% of the maximum vs is possible achieved in resting cell treatment with the salinity increased up to 5.0% NaCl in reaction media. Therefore, this Bacterial system has potential for dye decolorization bioprocesses occurring at high temperature and salt concentrations e.g. for cleaning dye-containing saline wastewaters.

Keywords

Azoreductase; Brilliant Black BN; Dermacoccus abyssi; Dye reduction; Mariana Trench.

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