1. Academic Validation
  2. Suppressive effects of irisflorentin on LPS-induced inflammatory responses in RAW 264.7 macrophages

Suppressive effects of irisflorentin on LPS-induced inflammatory responses in RAW 264.7 macrophages

  • Exp Biol Med (Maywood). 2014 Aug;239(8):1018-1024. doi: 10.1177/1535370214530081.
Yuan Gao 1 Lei Fang 1 Fen Liu 1 Chuanjie Zong 1 Runlan Cai 1 Xi Chen 1 Yun Qi 2
Affiliations

Affiliations

  • 1 Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
  • 2 Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China yqi@implad.ac.cn.
Abstract

Irisflorentin, a naturally occurring isoflavone, is an abundant active constituent in Rhizoma Belamcandae. Although some chemical studies have been reported, pharmacological actions of irisflorentin are not well studied. In this study, we demonstrate the anti-inflammatory activity of irisflorentin in lipopolysaccharides (LPS)-stimulated RAW 264.7 macrophages. Irisflorentin markedly reduces the transcriptional and translational levels of inducible nitric oxide synthase (iNOS) as well as the production of NO. Furthermore, it also significantly inhibits TNF-α, IL-1β and IL-6 at both the transcriptional and translational levels. These effects mainly act via ERK1/2 - and p38-mediated the activator protein-1 (AP-1) rather than the nuclear factor-κB (NF-κB) pathway. Thus, our study elucidates the anti-inflammatory mechanism of irisflorentin in LPS-activated RAW 264.7 macrophages.

Keywords

AP-1 pathway; Irisflorentin; LPS; RAW 264.7 macrophages; anti-inflammatory.

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